番茄红素对鱼藤酮所致PC12细胞线粒体损伤的影响  

Effect of Lycopene on Mitochondrial Damage of PC12 Cells Induced by Rotenone

作　　者：罗连响[1] 李晓玲[2] 朱少平[3] 曾敏娟[3] 鲍波[1,3] 

机构地区：[1]广东医学院病理生理教研室,广东湛江524023 [2]广东医学院微生物学和免疫学教研室,广东湛江524023 [3]广东医学院实验动物学教研室,广东湛江524023

出　　处：《中国实验方剂学杂志》2013年第20期181-185,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划项目(2010B060500015);广东医学院青年基金(XQ1309)

摘　　要：目的:研究番茄红素对鱼藤酮诱导PC12细胞线粒体损伤的保护作用。方法:采用鱼藤酮(0.5μmol·L-1)诱导PC12细胞损伤模型,给予番茄红素(3,10,30μmol·L-1)预处理2 h后,加入鱼藤酮,使终浓度达到0.5μmol·L-1,培养24 h后,CCK-8法检测细胞存活率,倒置相差显微镜观察细胞形态改变、透射电镜观察细胞线粒体超微结构,罗丹明123染色检测线粒体膜电位,以观察番茄红素的保护作用。结果:CCK-8结果显示与正常对照组比较,模型组细胞存活率下降为70.34%±2.81%(P<0.05),与模型组比较,番茄红素(3,10,30μmol·L-1)预处理后其细胞存活率分别提高到83.09%±3.15%,87.24%±2.15%,89.17%±2.26%(P<0.05);倒置相差显微镜观察显示鱼藤酮可导致PC12细胞凋亡,经番茄红素处理后,模型细胞凋亡情况明显改善;透射电镜观察显示鱼藤酮可导致PC12细胞线粒体超微结构发生改变,经番茄红素处理后,模型细胞凋亡情况明显改善;流式细胞仪检测结果显示与正常对照组比较,模型组细胞线粒体膜电位平均荧光强度下降为151.63±12.25(P<0.05),经番茄红素(3,10,30μmol·L-1)预处理后线粒体膜电位平均荧光强度分别提高到202.24±26.28,226.21±9.71,238.83±10.29(P<0.05)。结论:番茄红素对鱼藤酮诱导的PC12细胞线粒体损伤有保护作用。Objective：To investigate the protective effects of lycopene against rotenone-induced mitochondrial damage in PC12 cells.Method：All the experiments were carried out with PC12 in vitro,which were divided into5 group,control,model （0.5 μmol ·L-1）,lycopene （3,10,30 μmol·L-1） preincubation.Cell viability was determined by CCK-8 assay; inverted phase contrast microscope was used to observe the cell morphological was; the ultrastructural changes of neuronal mitochondria were viewed under transmission electron microscope.The flow cytometry was used to detect the mitochondrial membrane potential （MMP）.Result：CCK-8 assay showed that rotenone had cytotoxicity in PC12 cells,compared with the cells in the control group,cell viability was declined to 70.34％ ±2.81％ （P ＜0.05）,the pre-treatment of lycopene （3,10,30 μmol ·L-1）could significantly increase the PCI2 cell viability,cell viability was increased to 83.09％ ± 3.15％,87.24％ ±2.15％,89.17％ ± 2.26％ （P ＜ 0.05）.Compared with the cells in the model group,the number and morphology of neuronal mitochondria changed distinctly in the lycopene pretreated cells.Compared with the cells in the model group,the pre-treatment of lycopene （3,10,30 μ mol ·L-1） increased mean fluorescence intensity of mitochondrial membrane potential to 202.24 ± 26.28,226.21 ± 9.71,238.83 ± 10.29 （P ＜ 0.05）.Conclusion：Lycopene can exert protective effects against rotenone-induced mitochondrial damage in PC12 cells.

关 键 词：帕金森病 番茄红素 PC12细胞 鱼藤酮 线粒体 

分 类 号：R285.5[医药卫生—中药学]