表阿霉素纳米胶束靶向治疗大鼠移植性肝癌  

Epirubicin nanoparticles for targeting therapy of rat transplanted hepatocarcinoma

作　　者：李晨玉[1] 宋晓斌[1] 杨威[2] 刘铜军[3] 葛巨刚 景暇斌 

机构地区：[1]吉林省人民医院急诊外科,长春130021 [2]吉林大学第一医院 [3]吉林大学中日联谊医院 [4]松原市人民医院 [5]中国科学院长春应用化学研究所

出　　处：《中华实验外科杂志》2013年第10期2052-2055,共4页Chinese Journal of Experimental Surgery

基　　金：吉林省科技厅资助项目（20130206033SF）

摘　　要：目的 探讨键合表阿霉素纳米胶束（EPI-NPs）在大鼠移植性肝癌模型肝内的分布及药效.方法 制备具有靶向控释作用的EPI-NPs,Walker-256细胞直接注射法制作大鼠移植性肝癌模型,通过肝动脉灌注给药（EPI含量均为2 mg/kg）,利用组织荧光和匀浆荧光技术对比分析EPI-NPs和表阿霉素（EPI）在肝内的分布特点,并作药效对比.结果 EPI-NPs组肿瘤组织的EPI荧光强度高于肝组织（P＜0.05）,而EPI组则表现为平均分布；EPI-NPs组在肿瘤及肝内的EPI荧光强度均高于EPI组（P＜0.05）.EPI-NPs组大鼠肝移植瘤的体积抑瘤率及质量抑瘤率分别为72.96％和68.19％,EPI组的体积抑瘤率及质量抑瘤率分别为39.16％和35.33％,两者差异均有统计学意义（P＜0.05）.EPI-NPs组生存率高于EPI组（P＜0.05）.结论 EPI-NPs具有良好的肿瘤组织靶向性和抑瘤效果.Objective To study the distribution and anti-tumor effect of epirubicin nanoparticles （EPI-NPs） in transplanted hepatocarcinoma rat model.Methods EPI-NPs with targeting and controlled release quality were prepared.The transplanted hepatocarcinoma rat models were made by direct injection of cancerous ascites with Walker-256 cells,and the drug was delivered to the rat model through hepatic arterial infusion （EPI content was 2 mg/kg）.The distribution of EPI-NPs in the hepatocarcinoma rat model was observed by tissue fluorescence analysis and homogenate fluorescence analysis.The anti-tumor effect of EPI-NPs was also studied.Results The intensity of drug fluorescence in tumor tissue was stronger in EPI-NPs group than that in normal tissue at different time points （P ＜ 0.05）.As to the EPI group,the fluorescence distributed equably in tumor and normal tissue.The intensity of drug fluorescence in tumor and intrahepatic tissue of EPI-NPs group was stronger than in EPIs group （P ＜ 0.05）.The volume anti-tumor ratio and the quality anti-tumor ratio in EPI-NPs group were 72.96％ and 68.19％ respectively,which were 39.16％ and 35.33％ in EPI group （P ＜0.05）.The survival rate in EPI-NPs group was higher than that in EPI group.Conclusion EPI-NPs were proved to have good tumor targeting essence and favourable tumor suppression effect.

关 键 词：表阿霉素 移植瘤 癌 肝细胞 纳米 

分 类 号：R735.7[医药卫生—肿瘤]