条件性敲除Osterix基因获得先天性脊柱侧凸小鼠动物模型及表型分析  

Generation of congenital scoliosis mouse model with Osterix conditional knockout and initial phenotype analysis

作　　者：陈思旭[1] 宗兆文[1] 贾敏[1] 沈岳[1] 赵玉峰[1] 郭庆山[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所创伤科创伤、烧伤与复合伤国家重点实验室,重庆400042

出　　处：《中华实验外科杂志》2013年第10期2169-2171,F0004,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81271935）;创伤、烧伤与复合伤国家重点实验室自主课题项目（SKLZZ201124）;＂十二五＂科研项目重大项目分课题项目（AWS11J008）

摘　　要：目的 通过Osterix基因敲除获得先天性脊柱侧凸动物模型及表型分析.方法 应用Cre-LoxP系统繁育成骨细胞特异性敲除Osterix基因小鼠.取4、12周龄的敲除小鼠各10只,以同龄同系野生型小鼠各10只为对照,行全身及脊柱X线摄像观察；收集脊柱标本行苏木素-伊红（HE）染色及抗酒石酸酸性磷酸酶（TRAP）染色观察并检测.结果 成功获得成骨细胞特异性敲除Osterix基因小鼠,X线显示敲除小鼠中75％出现严重的脊柱侧凸,Cobb角平均值为35..HE染色显示敲除小鼠椎体生长板增宽,椎体骨量增加,TRAP染色显示腰椎中破骨细胞数量显著减少（P＜0.05）.结论 小鼠成骨细胞中条件性敲除Osterix基因后成功获得先天性脊柱侧凸模型,提示Osterix在先天性脊柱侧凸的发病中有重要作用.Objective To obtain a congenital scoliosis mouse model with Osterix conditional knockout in osteoblasts and initial phenotype analysis.Methods We adapted the Cre-LoxP recombination system to create the Osterix conditional knockout mice.X-ray radiology and HE staining were used to evaluate the change of vertebral form and bone volume,and tartrate resistant acid phosphatase staining （TRAP） staining was used to assess the activity of osteoclasts in both Osterix knockout and wild-type mice （4 weeks and 12 weeks old,n =10 each）.Results Osterix conditional knockout mice were successfully obtained.X-ray examination showed that about 75％ of all the Osterix conditional knockout mice exhibited congenital scoliosis phenotypes,and the mean value of cobb angle was 35°.Hematoxylin and eosin （HE） staining showed that the bone volume in the lumbar vertebral body was increased significantly in Osterix knockout mice.TRAP staining showed that the number of osteoclasts was decreased in Osterix knockout mice （P ＜ 0.05）.Conclusion A congenital scoliosis mouse model was obtained with Osterix conditional knockout in osteoblasts,which implies that Osterix plays an important role in the etiology of congenital scoliosis.

关 键 词：OSTERIX 先天性脊柱侧凸 条件性基因敲除 模型 动物 

分 类 号：R-332[医药卫生]