猪控制型心死亡供体模型的建立  

Establishment of a porcine model of controlled donors of cardiac death

作　　者：郭明晓[1] 姚丹华[1] 孔文成[1] 李幼生[1] 黎介寿[1] 

机构地区：[1]南京大学医学院临床学院南京军区南京总医院解放军普通外科研究所,210002

出　　处：《中华实验外科杂志》2013年第10期2218-2220,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81072438）

摘　　要：目的 建立猪控制型心死亡供体模型,观察模型建立过程中血流动力学及动脉血气指标的变化.方法 白色杂种猪10只,麻醉成功后静脉给予阿曲库铵（1 mg/kg）及肝素（150 ～200 U/kg）,同时撤除呼吸机以建立心死亡模型,监测模型建立过程中血压、心率、平均动脉压以及动脉血气（pH值、PaO2、PaCO2以及乳酸）的变化.结果 心死亡时间为（17.40±3.37） min.撤除呼吸机5 min内,猪出现短暂升高血压、心动过速,收缩压由（113.90±11.52） mmHg（1 mmHg=0.133 kPa）升至（118.50±10.40） mmHg,心率由（108.20±4.94）次/分升至（154.90±7.06）次/分,之后血压、心率迅速降低直至心跳停搏；同时机体动脉氧分压迅速降至（17.40±3.13） mmHg,乳酸水平升高,酸中毒加重.结论 通过模拟临床控制型心死亡供体“撤除呼吸机、机体肝素化、确定死亡时间”3个阶段,成功建立了稳定、可靠、重复性高的猪心死亡模型.Objective To establish a porcine model of controlled donors of cardiac death （CDCD） and investigate the changes of hemodynamics and arterial blood gas.Methods Ten domestic crossbred pigs were anesthetized and ventilated with 100％ oxygen.Once all baseline data were taken,atracurium （1 mg/kg） was administered and the ventilator was discontinued while the animal was under deep anesthesia to establish the porcine CDCD model.Meanwhile,heparin （150-200 U/kg） was administered after discontinuation of the ventilator.The changes of blood pressure,heart rate,average blood pressure and arterial blood gas were monitored every 5 min until cardiac death.Results The time to death was approximately （17.40 ±3.37） min.Within 5 min of removal of the ventilator,systolic blood pressure and heart rate were quickly increased to （118.50 ± 10.40） bpm and （108.20 ±4.94） bpm respectively.Then blood pressure and heart rate were rapidly reduced until the heart cardiac arrest.Blood gases throughout the apneic time showed a rapid hypercapnia and acidosis.Conclusion A new swine CDCD model,simulating three stages of ＂withdrawal of ventilation,systemic anticoagulation and determination of death＂,has been successfully established.

关 键 词：心脏死亡供体 控制型供体 猪 

分 类 号：R-332[医药卫生]