啶菌噁唑Z、E异构体的拆分及其抑菌活性比较  

Separation of SYP-Z048 Z-,E- isomers and evaluation on their inhibitory activities to fungi

作　　者：黄娅蓝[1] 宁明宇[2] 刘鹏飞[1] 黄中乔[1] 刘君丽[3] 刘西莉[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]全国农业技术推广服务中心种业信息与技术处,北京100125 [3]沈阳化工研究院有限公司新农药创制与开发国家重点实验室,沈阳110021

出　　处：《植物病理学报》2013年第5期549-555,共7页Acta Phytopathologica Sinica

基　　金：公益性行业(农业)科研专项经费资助(201303025);国家自然科学基金青年基金项目资助(31000866)

摘　　要：啶菌（噁）唑是一个新型异（噁）唑类广谱杀菌剂,为探索其异构体的抑菌活性差异,采用薄层层析、柱层析和高效液相色谱法对啶菌（噁）唑顺反异构体进行了分离和纯度检测,并测定了其对担子菌门、子囊菌门和无性型真菌类14个属16株植物病原真菌的离体活性.试验分离获得了纯度分别为94.9％和95.0％的啶菌恶唑Z、E异构体.离体条件下,Z异构体对供试病原真菌EC50值范围为0.000 2～0.730 3μg/mL,E异构体EC50值范围为0.003 1 ～ 49.182 7μg/mL,Z异构体对14株病原菌抑菌活性显著（P＜0.01）高于E异构体.SYP-Z048 is a new isoxazolidine fungicide targeting a wide range of plant diseases.To separate its isomers and evaluate their fungicidal activity,thin layer chromatography （TLC）,column chromatography,high performance liquid chromatography （HPLC） and bioassay analysis were conducted.Z-,E-isomers were separated and the purities were determined as 94.9％ and 95.0％.In vitro,both of the isomers inhibited the mycelial growth of 16 experimented fungi from 14 genera.The results showed that the EC50 values of Z-isomer ranged from 0.000 2 to 0.730 3 μg/mL and the EC50 values of E-isomer ranged from 0.003 1 to 49.182 7 μg/mL.For 14 pathogens,the Z-isomer was significantly （P ＜0.0l） more effective than E-isomer.

关 键 词：啶菌(噁)唑 异构体 抑菌活性 高效液相色谱 

分 类 号：S482.2[农业科学—农药学]