铝对荞麦根系DNA损伤的效应研究  

Effect of Buckwheat Root DNA Damage on Aluminum

作　　者：沈丹艳[1] 梅笑漫[2] 姚巧美[1] 江心怡[1] 蔡梦颖[1] 吴凤清[1] 范泱 张白[1] 高培培[1,3] 刘鹏[1,3] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]杭州师范大学初等教育学院,浙江杭州330036 [3]浙江师范大学生态研究所,浙江金华321004

出　　处：《水土保持学报》2013年第5期244-248,共5页Journal of Soil and Water Conservation

基　　金：国家自然科学基金项目(30970188;30770161);浙江省公益技术研究农业项目(2011C22053);浙江省自然科学基金项目(Y307408)

摘　　要：以荞麦(Fagopyrum esculentum)为材料,用不同浓度的铝进行土培,研究铝对荞麦根系DNA的损伤效应。结果表明:(1)当铝浓度低于0.45g/kg时,荞麦的总根长增加,根系活力上升,对荞麦生长有一定的促进作用;当铝浓度达到0.6g/kg时,荞麦的根长变短,根系活力下降,不利于荞麦的生长发育。(2)不同浓度铝处理后,荞麦根系DNA呈现出不同程度的增色效应。(3)通过CASP软件对彗星电泳实验图像进行分析显示,随着铝浓度的增加,彗星头部渐小,拖尾逐渐加长。研究结果还表明:铝处理通过对荞麦根系生长产生影响,还对其根系DNA造成损伤,但是从增色效应和彗星电泳的结果可以看出,铝对荞麦所产生的损伤并不是很明显,综合荞麦根系DNA的研究也表明,荞麦是一种耐铝的植物。Using buckwheat（Fagopyrum esculentum）as the materials,the root DNA damage of buckwheat was studied by different aluminum concentrations on soil culture.The results show that：（1）Low concentration（not exceeding 0.45g/kg）of aluminum could not only increase the total root length and root vigor of buckwheat,but also promote the growth of buckwheat.High concentration of aluminum（0.6g/kg）declined the root length and root vigor of buckwheat,and inhibited the growth and development of buckwheat.（2）Under different concentration of aluminum treatment,root DNA of buckwheat presented different degrees of hyperchromic effect.（3）Through analysis of the CASP software,the comet head was gradually becoming small and trailing was gradually extended as aluminum concentration aggravates.The results also showed that aluminum processing had effect on root growth of buckwheat and caused root DNA damage of buckwheat.But it could be seen from the hyperchromic effect and comet electrophoresis results,aluminum on buckwheat damage was not obvious.Comprehensive buckwheat root DNA study also showed that buckwheat was a kind of plant resistance to aluminum.

关 键 词：铝 荞麦 根系DNA损伤 增色效应 彗星电泳 

分 类 号：S143.72[农业科学—肥料学] Q946.2[农业科学—农业基础科学]