β-环糊精交联聚合物与盐酸巴马汀包合作用的荧光光谱研究及其分析应用  

Spectrofluorimetric Study of Inclusion Interactions of β-cyclodextrins Cross-linked Polymer with Palmatine Chloride and Its Analytical Application

作　　者：郭京波[1,2] 赵郭燕[1] 张利兵[1] 杜黎明[1] 

机构地区：[1]山西师范大学分析测试中心,山西临汾041004 [2]山西师范大学工程学院,山西临汾041004

出　　处：《分析科学学报》2013年第5期665-668,共4页Journal of Analytical Science

基　　金：教育部高等学校博士学科点专项科研基金(No.20091404110001)

摘　　要：采用荧光光谱法研究了β-环糊精交联聚合物(β-CDP)与盐酸巴马汀(PAL)的相互作用。结果表明,在强碱性条件下β-CDP对PAL能产生显著的荧光增敏作用,据此建立了水溶液中高灵敏测定PAL的荧光光谱法。在最佳条件下,适量PAL溶液,1mL 0.001mol/L的β-CDP水溶液,6.0mL 10mol/L NaOH溶液,室温下反应,24h内完成测定,测定β-CDP-PAL包合物荧光强度的激发波长364nm,发射波长442nm。测定PAL的线性范围为2～1 000ng/mL,检出限为0.65ng/mL。该方法可用于药物制剂及生物体液中PAL含量的测定。The binding capacity ofβ-cyclodextrins cross-linked polymer（β-CDP） to palmatine chloride （PAL） has been studied by spectrofluorimetry. The results showed that with PAL in 6.0 mol/L NaOH β-CDP formed stable inclusion complex, meanwhile the fluorescence intensity of PAL increased remarkably. At the optimum reaction conditions, the linear calibration graph were obtained in the concentration range of 2-1 000 ng/mL for the investigated samples and the detection limit was 0.65 ng/mL. The proposed method was applied successfully for the determination of PAL in pharmaceutical dosage form, spiked human urine and spiked plasma.

关 键 词：盐酸巴马汀 Β-环糊精交联聚合物 荧光光谱 

分 类 号：O657.3[理学—分析化学]