荧光光谱法研究姜黄素与β-环糊精及其衍生物包合作用  

Study on the Inclusion Interaction of Curcumin and β-cyclodextrin and Its Derivatives by Fluorescence Spectrometry

作　　者：周叶红[1] 武宏娟[1] 樊丽[1] 马静[2] 双少敏[1] 

机构地区：[1]山西大学环境科学研究所,山西太原030006 [2]山西医科大学,山西太原030001

出　　处：《分析科学学报》2013年第5期673-676,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.21175087);山西医科大学学生创新基金(No.201231)

摘　　要：采用荧光光谱法,在室温下研究了pH=2.02的磷酸盐缓冲溶液中β-环糊精(β-CD)、甲基-β-环糊精(M-β-CD)、羟丙基-β-环糊精(HP-β-CD)、磺丁基-β-环糊精(SBE-β-CD)对姜黄素的包合作用。固定姜黄素浓度,逐渐改变β-CD及其衍生物的浓度,姜黄素的荧光强度逐渐增强,表明包合物的形成,同时用荧光双倒数法计算了β-CD及其衍生物与姜黄素的包合常数。结果表明,室温下,在pH=2.02的磷酸盐缓冲体系中,SBE-β-CD对姜黄素的包合能力最强,四种环糊精与姜黄素的包合比均为1∶1。初步探讨了SBE-β-CD荧光增敏作用对姜黄素的定量测定,线性范围为5.0×10-7～4.5×10-6 mol/L,检出限为1.2×10-8 mol/L。The inclusion interactions of curcumin and β-cyclodextrin（β-CD）,methyl-β-cyclodextrin（M-β- CD）, hydroxypropyl-β-cyclodextrin （HP-β-CD） and sulfobutylether-β-cyclodextrin （SBE-β-CD） were investigated by fluorescence spectroscopy at room temperature. The fluorescence of curcumin was enhanced remarkably with the addition of CDs, which indicated the formation of inclusion compounds. And the inclusion constants of inclusion compounds were calculated by fluorescence spectrometric method. The results showed that at room temperature, SBE-β-CD has the strongest inclusion capacity in pH =2.02 phosphate buffer. And the inclusion ratio were all 1 ： 1 between curcumin and CDs. The SBE-β-CD fluorescence sensitization effect was used for the quantitative determination of curcumin. The linear range was5.0×10 7-4.5×10-7 mol/L,the detection limit was 1.2×10-8 mol/L.

关 键 词：姜黄素 Β-环糊精及其衍生物 荧光光谱 包合作用 

分 类 号：O657.39[理学—分析化学]