甘珀酸对EAE小鼠IL-12、IL-23表达的影响  

Effects of Carbenoxolone on the Expressions of IL-12 and IL-23 in EAE Mice

作　　者：孙叶飞[1] 张旭[2] 曲慧玲[3] 李恩东[4] 赵传胜[3] 

机构地区：[1]中国医科大学附属第一医院胃肠外科,沈阳110001 [2]中国医科大学基础医学院解剖学教研室,沈阳110001 [3]中国医科大学附属第一医院神经内科,沈阳110001 [4]日本名古屋大学大学院神经免疫学教研室

出　　处：《中国医科大学学报》2013年第10期903-906,共4页Journal of China Medical University

基　　金：辽宁省自然科学基金(201202276)

摘　　要：目的探讨甘珀酸(CBX)对小鼠实验性自身免疫性脑脊髓炎(EAE)IL-12、IL-23表达的影响。方法-制作小鼠EAE模型,然后将CBX(20mg/kg)或生理盐水隔日1次静脉注射,比较各组行为学变化。小胶质细胞与树突细胞与阶梯浓度的CBX共培养后,用RT-PCR检测IL-12和IL-23-mRNA的表达。用ELISA检测IL-12与IL-23水平。结果 CBX组和EAE组比较,行为学表现有所好转(P<0.05)。体外实验证实与LPS组比较,CBX组小胶质细胞及树突细胞IL-23-p19的表达降低(P<0.05),同时CBX处理后能够抑制小胶质细胞IL-23水平(P<0.05)。结论 CBX可通过抑制小胶质细胞或树突细胞IL-23的表达改善多发性硬化的临床症状。Objective To investigate the effects of carbenoxolone on IL- 12 and IL-23 expression in EAE mice. Methods The EAE models were established using MOG35-55, and then the mice were intravenously administered with CBX （20 mg/kg） or vehicle control （saline） every other day with the first dose on the day of immunization. The changes of behavioral changes were recorded. Mieroglia and dendritic cells were incubated with 1 p,g/mL IX＇S in the presence of a graded concentration of CBX for 24 h. The IL- 12 mRNA and IL-23 mRNA expressions were detected by semiquanti- tative RT-PCR. Supernatants were collected after an additional 24 h culture for ELISA, and IL- 12 and IL-23 levels were measured. Results Behav- ioral changes in CBX group were improved compared with EAE group （P 〈 0.05 ）. CBX negatively regulated the expressions of IL-23 p19 in microg- lia and dendritic cells. IL-23 production by microglia was decreased in CBX group. Condusion The level of IL-23 in microglia and dendritic cells was negatively regulated by CBX, and CBX can improve the multiple sclerosis clinical symptoms.

关 键 词：甘珀酸 多发性硬化 IL-12 IL-23 

分 类 号：R741[医药卫生—神经病学与精神病学]