不同浓度二甲双胍对子宫内膜癌细胞增殖与凋亡的影响  

Effect of metformin on proliferation and apoptosis of human endometrial carcinona cells

作　　者：宋红林[1] 梁少凤[1] 

机构地区：[1]广西医科大学附属肿瘤医院妇瘤科,南宁530021

出　　处：《中国临床新医学》2013年第8期739-741,共3页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：广西卫生厅重点科研课题(编号:桂卫重200972)

摘　　要：目的探讨二甲双胍对子宫内膜癌细胞系Ishikawa细胞增殖、凋亡和细胞周期的影响。方法以不同浓度及时间二甲双胍作用Ishikawa细胞,采用四甲基偶氮唑蓝比色法和流式细胞仪检测细胞增殖、凋亡和细胞周期。结果二甲双胍抑制子宫内膜癌细胞增殖,并与作用时间浓度相关。流式细胞仪检测提示二甲双胍作用下细胞中G0/G1期细胞比例增加,S期细胞比例降低。结论二甲双胍能抑制子宫内膜癌Ishikawa细胞增殖,使细胞生长周期停滞。Objective To investigate the effect of metformin on proliferation,cell cycle distribution and apoptosis of human endometrial carcinoma cell lines in vitro.Methods The effects of metformin on proliferation,cell cycle distribution and apoptosis of Ishikawa cell lines were measured by MTT assay and fluorescence-activated cell sorting technique.Results Metformin potently inhibited growth in a dose-dependent manner in Ishikawa cell lines in a dose-and time-dependent fashion.Flow cytometric analysis showed that metformin increased the number of cells in G0/G1 and reduced the percentage of cells in S phase.Conclusion Metformin is a potent inhibitor of cell proliferation in endometrial cancer cell lines.

关 键 词：子宫内膜肿瘤 二甲双胍 细胞增殖 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]