普洱毛脚乌鸡与南涧绿耳乌鸡线粒体DNA母系遗传分析  

Maternal Genetic Analysis of Puer Miaojiao and Nanjian Lver Black-bone Chicken Using mtDNA D-loop Sequences

作　　者：苗永旺[1] 孙利民 童晶晶[1] 李大林 袁跃云 杨满灿[1] 郝伟峰[1] 霍金龙[1] 杨润 余仕亮 杨光强 王群 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南省家畜改良工作站,云南昆明650225 [3]云南省普洱市畜牧工作站,云南普洱思茅区665000 [4]云南省景东县畜牧工作站,云南景东676200

出　　处：《家畜生态学报》2013年第7期10-14,共5页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金项目(30660024);云南省教育厅科学研究基金项目(5Y0196B;0112340);云南省应用基础研究重点项目(2007C0003Z);云南省应用基础研究计划面上项目(2006C0034M)

摘　　要：为了从母系遗传角度阐明普洱毛脚乌鸡与南涧绿耳乌鸡的遗传差异,本研究采用PCR产物直接测序法对随机采集的48只普洱毛脚乌鸡和48只南涧绿耳乌鸡的mtDNA控制区第I高变区序列进行了群体变异检测并对获得的数据进行了群体遗传和系统发育分析。在普洱毛脚乌鸡中检测到24个核苷酸多态位点,共定义了12种单倍型,单倍型多样度为0.829±0.039,群体内遗传距离为0.016±0.003。在南涧绿耳乌鸡中共检测到28个核苷酸多态位点,共定义了13种单倍型,单倍型多样度为0.907±0.018,群体内遗传距离为0.016±0.003。系统发育分析显示:普洱毛脚乌鸡包含A、B、E、F和G五个母系血统,它们所占的比例分别为45.83%、2.08%、8.33%,16.67%和27.09%。而南涧绿耳乌鸡具有A、B、C、E、F和G六个母系血统,它们占的比例分别为22.92%、14.58%、4.17%、10.42%、16.67%和31.25%。结果表明,南涧绿耳乌鸡的mtDNA遗传多样性相对较高,两地区乌骨鸡的群体遗传组成存在明显差异;它们在血统上主要为地方土鸡血统,但可能有少量商品鸡血液渗入。In order to elucidate the genetic differences between Puer Miaojiao and Nanjian Lver blackbone chicken,the hypervariable region Ⅰ sequence variations in mtDNA D-loop of the respective 48 random samples from Puer Miaoiiao and Nanjian Lver black-bone chicken were examined by direct sequencing of PCR products in the present study,and an analysis of population genetics and phylogenetics were conducted on the data obtained.In Puer Miaojiao black-bone chicken,a total of 24 polymorphic sites included 2 singleton polymorphic sites and 24 parsimony informative sites were detected,which defined 12 haplotypes and the unique haplotypes accounted for 50％.The haplotype diversity,nucleotide diversity and the average genetic distance within the population were 0.829 ± 0.039,0.01559±0.00068 and 0.016 ±0.003,respectively.In Nanjian Lver black-bone chicken,a total of 28 polymorphic sites included 0 singleton polymorphic site and 28 parsimony informative sites were detected,which defined 13 haplotypes and the unique haplotypes accounted for 53.85％.In this population,the haplotype diversity,nucleotide diversity and the average genetic distance within the population were 0.907±0.018,0.01590±0.00056 and 0.016±0.003,respectively.Phylogenetic and genetic divergence analysis revealed that there were A,B,E,F and G five matrilineages in Puer Miaojiao black-bone chicken which accounted for 45.83％,2.08％,8.33％,16.67％ and 27.09％,respectively.And there were A,B,C,E,F and G six matrilineages in Nanjian Lver blackbone chicken which accounted for 22.92 ％,14.58 ％,4.17％,10.42 ％,16.67 ％ and 31.25 ％,respectively.The results in this study indicated that the population genetic variation in Nanjian Lver black-bone chicken are higher than that in Puer Miaojiao black-bone chicken and the population genetic composition for the two chicken populations were significantly different.They are mainly for the native chicken descent by birth,but there may be a small amount of commercial chicken blood mixed.

关 键 词：普洱毛脚乌鸡 南涧绿耳乌鸡 MTDNA D-LOOP 遗传多态性 遗传差异 

分 类 号：S811.6[农业科学—畜牧学]