绵羊X染色体59194976位点多态分析及其与尾(臀)脂性状相关性研究  

Polymorphism Analysis of the Position(59194976) on Sheep X Chromosome and Its Relationship with Fat-tail(Fat-rump) Trait

作　　者：王世银[1,2] 张伟[1,2,3] 沈敏[1] 李欢[1] 梁耀伟[1] 杨井泉[1] 高磊[1] 甘尚权[1] 王新华[1] 

机构地区：[1]新疆农垦科学院畜牧兽医研究所,石河子832000 [2]新疆农业职业技术学院,昌吉831100 [3]石河子大学动物科技学院,石河子832000

出　　处：《基因组学与应用生物学》2013年第5期575-580,共6页Genomics and Applied Biology

基　　金：国家973前期专项(2011CB111501);国家自然科学基金委-新疆联合重点项目(U1130302)共同资助

摘　　要：尾脂沉积能力在脂尾型绵羊和瘦尾型绵羊间存在很大的差异,但其沉积脂肪的遗传特性与分子机制仍不明晰。为此,本研究选择尾型极端差异的阿勒泰羊(巨型脂臀)、湖羊(短脂尾)和黑萨福克羊(长瘦尾),采用PCR-RFLP方法检测绵羊X染色体59194976位点多态性,分析其与尾(臀)脂沉积能力的相关性。结果表明:在阿勒泰羊群体中G等位基因属优势等位基因,其两等位基因比值(G/A)分别是湖羊和黑萨福克羊的6倍和68倍,且G等位基因在阿勒泰羊中趋于纯合。卡方检验结果表明,阿勒泰羊在该位点均处于HardyWeinberg平衡状态(p>0.05),而湖羊和黑萨福克羊在该位点均处于Hardy-Weinberg极不平衡状态(p<0.01)。以上结果提示,绵羊X染色体59194976位点SNP可作为理想的分子标记应用于高、低脂绵羊品种选育。The ability of fat deposition has a great difference between the fat-tail and thin-tail sheep, but the heredity and molecule mechanism of fat accumulation is poorly understood. In recent documents, the single nucleotide polymorphsim （SNP） locus at the 59194976 nucleotide of X chromosome was reported as a moleculer marker of fat accumulation. In this study, using PCR-RFLP method, the Genotypes and allele frequencies distribu- tions was analyzed in three sheep breeds ofAltay, Hu and Suffolk, which there are extremely difference on tail type. The result showed that G allele gene of 59194976 locus is preponderant allele gene in the Altay which is fat-tail or fat-rump sheep breed, the ratio of two allele gene （G/A） is 6 and 68 times compared with Hu and Suffolk sheep, and the G allele gene has a homozygosity trend in Altay sheep. The result of chi-square analysis suggested that the allele frequencies and genotype frequencies were in Hardy-Weinberg equilibrium （p〉0.05） in Altay sheep, but were not in Hardy-Weinberg equilibrium （P〈0.01） in Hu and Suffolk sheep. This study demonstrated that the SNP locus of 59194976th on X chromosome was a perfect molecular marker of sheep breeding in high or low fat.

关 键 词：脂臀性状 多态性 等位基因 基因型 分子标记 

分 类 号：S826[农业科学—畜牧学]