橡胶树魏克汉种质群体结构分析  

Population Structure Analysis of Wickham Germplasm in Hevea brasiliensis

作　　者：方家林[1] 龙青姨[2] 华玉伟[1] 黄华孙[1] 

机构地区：[1]中国热带农业科学院橡胶研究所,农业部橡胶树生物学与遗传资源利用重点实验室,儋州571737 [2]云南省热带作物科学研究所,景洪666100

出　　处：《基因组学与应用生物学》2013年第5期627-632,共6页Genomics and Applied Biology

基　　金：国家天然橡胶产业技术体系(nycytx-34);农业部物种保护项目(NB2013-2130135和13RZZY-1)共同资助

摘　　要：群体结构是引起关联分析假阳性的最主要因素。本研究基于25个EST-SSRs位点,分析了283份橡胶树魏克汉种质群体的哈迪-温伯格平衡和群体分层情况。结果表明,在被检查的25个位点中13个位点偏离哈迪-温伯格平衡;在共祖系数为50%、60%、70%和80%时,283份种质中具有明确亚群归属的分别只有155份、110份、61份和22份,分别占总种质的54.78%、38.87%、21.55%和7.78%。同时,25个多态位点共形成了300个位点组合,20个位点组合(6.67%)呈显著的连锁不平衡状态,其中5个位点组合(1.67%)达到极显著连锁不平衡。由上可知283份橡胶树魏克汉种质所构成的群体具有一定的群体分层,但分化程度较小,可用于关联分析。Population stratification is key reason resulting in false association in association analysis. Analysis of Hardy-Weinberg equilibrium and population structure on 283 Wickham germplasm was carried out by 25 EST-SSRs. The result showed that 13 of 25 EST-SSRs showed that there were Hardy-Weinberg equilibrium, and 54.78%, 38.87%, 21.55% and 7.78% of entire Wickham germplasm could be of population stratification, additionally, 20 locus combinations （6.67%） of 300 EST-SSRs combinations-derived 25 EST-SSRs were significantly linkage dis- equilibrium. So there was genetic diversity of 283 Wickham germplasm but the level of genetic diversity was lower, the population could be used for association study.

关 键 词：橡胶树 魏克汉种质 EST-SSRS 群体结构 关联分析 

分 类 号：S794.1[农业科学—林木遗传育种]