云南白药和碘仿在大鼠腭裂手术中的应用及效果比较  

Comparison of curative effects between Yunnan Baiyao and iodoform applied in rats after cleft palate operation

作　　者：梁乃银 邹永巍[2] 张红玲[1] 

机构地区：[1]吉林大学口腔医院急诊科,吉林长春130021 [2]吉林大学口腔医院口腔颌面外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2013年第5期893-897,I0003,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(20030539)

摘　　要：目的:探讨在大鼠腭裂手术术中和术后应用云南白药和碘仿对伤口愈合的效果,寻找更适合于大鼠腭裂手术术中和术后应用的药物。方法:将90只SD大鼠随机分为对照组、碘仿组和云南白药组,每组30只。通过手术方法在大鼠硬腭部制作腭裂缺损,并根据分组在缺损中填塞云南白药或碘仿4mg,对照组不填药物。术后应用肛门测温法测定大鼠体温,应用骨密度仪检测腭部手术区骨密度,HE染色和改良gomori特殊染色法观察新骨形成情况,免疫组织化学法检测碱性成纤维细胞生长因子(bFGF)和骨形态发生蛋白2(BMP2)表达水平。结果:术后1周碘仿组大鼠体温高于其他2组(P<0.05);术后第2、3周X射线片显示云南白药组骨密度高于其他2组(P<0.05);HE染色及改良Gomori特殊染色观察到云南白药组成纤维细胞分裂、增殖和新骨形成情况明显强于对照组和碘仿组;术后第2、3周云南白药组bFGF和BMP2表达水平高于其他2组(P<0.05)。结论:腭裂手术大鼠应用云南白药对体温无刺激性升高,而且能够促进成纤维细胞增殖,减少术后腭瘘并发症的发生,同时能够促进腭裂的骨性修复。Objective To study the effects of Yunnan Baiyao and iodoform for rat cleft palate operation,and to find more suitable drug for the cleft palate operation.Methods 90SD rats were randomly divided into control group,drug iodoform group,and Yunnan Baiyao group;there were 30rats in each group.Through the method of operation,the rat cleft palate defects models were set up.According to the grouping,the 4mg Yunnan Baiyao or 4mg iodoform werer put into the defects,and nothing in control group.The body temperature of the rats was detected with anal temperature measurement.Bone density meter was used to detect the palate operation area bone density.HE staining and modified Gomori staining were used to observe new bone formation.The contents of bFGF and BMP2were determined by immunohistochemical method.Results The body temperature of the rats in iodoform group was higher than those in the other two groups 1 week after operation（P0.05）.The X-ray detection results showed that the bone density in Yunnan Baiyao group was higher than those in the other two groups 2and 3weeks after operation（P0.05）.The fibroblast division,proliferation and new bone formation were enhaced in Yunnan Baiyao group by HE and modified Gomori special staining.The contents of bFGF and BMP2in Yunnan Baiyao group were higher than those in the other two groups 2and 3weeks after operation（P 0.05）.Conclusion Yunnan Baiyao has no irritation to temperature,and it can not only promote the cleft palate soft tissue healing,but also reduce palatal fistula complications,and it can promote the bony repair of cleft palate.

关 键 词：大鼠 Sprague-Dawley 腭裂 云南白药 碘仿 骨性愈合 

分 类 号：R782.2[医药卫生—口腔医学]