子宫内膜癌患者外周血细胞CK20 mRNA的表达及其临床意义  

Expression of CK20 mRNA in peripheral blood cells of patients with endometrial cancer and its clinical significance

作　　者：王文越[1] 黄文君[2] 潘颖[1] 李析茜[1] 曹林林[3] 张纪存 杨麒巍 

机构地区：[1]吉林大学中日联谊医院妇产科,吉林长春130033 [2]吉林大学第二医院血液科,吉林长春130041 [3]吉林大学中日联谊医院中心研究室,吉林长春130033 [4]沈阳军区总医院心外科,辽宁沈阳110016

出　　处：《吉林大学学报（医学版）》2013年第5期960-964,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省卫生厅国际合作项目资助课题(20090747)

摘　　要：目的:检测子宫内膜癌患者外周血细胞中细胞角蛋白20(CK20)基因mRNA表达,探讨其表达对子宫内膜癌微转移的诊断价值。方法:采用实时荧光定量PCR(RT-qPCR)技术检测44例子宫内膜癌患者、34例子宫良性病变患者和32名健康志愿者外周血细胞中CK20mRNA表达水平,分析其表达与子宫内膜癌患者病理学参数之间的关系。结果:44例子宫内膜癌患者外周血细胞CK20mRNA检测出阳性12例,阳性率为27.3%;子宫良性病变患者及健康女性外周血中均未见其表达,子宫内膜癌患者CK20mRNA阳性表达率高于子宫良性病变患者及健康女性(χ2=20.204,P<0.05);子宫内膜癌患者外周血细胞CK20mRNA表达水平与临床分期(χ2=16.712,P<0.05)、淋巴结转移(χ2=6.381,P<0.05)和浸润深度(χ2=6.381,P<0.05)存在关联性,而与病理分级(χ2=0.229,P>0.05)及是否绝经(χ2=0.004,P>0.05)无关联。结论:相对于子宫良性病变患者及健康女性,CK20mRNA在子宫内膜癌患者中阳性表达率明显升高,且表达水平与临床分期、是否有淋巴结转移及浸润深度有关;CK20的特异性强,可作为判断子宫内膜癌血源性微转移的参考指标。Objective To detect the expression of CK20gene mRNA in peripheral blood cells of the patients with endometrial cancer,and to explore its diagnostic value of the CK20 gene expression in endometrial cancer micrometastasis.Methods The expressions of CK20gene mRNA in peripheral blood cells in 44patients with endometrial cancer,34patients with uterine benign diseases and 32healthy volunteers were detected by real-time fluorescence quantitative PCR（RT-qPCR）.The relationship between its expression and the pathological parameters of the patients with endometrial cancer was analyzed.Results The positive expression rate of CK20 mRNA in peripheral blood cells in 44patients with endometrial cancer was 27.3%（12/44）;there was no expression of CK20 mRNA in the uterine benign diseases patients and healthy women;the positive expression rate of CK20mRNA in the patients with endometrial cancer was higher than those in the patients with uterine benign diseases and healthy women（χ2=20.204,P0.05）.The expression level of CK20mRNA had positive correlation with clinical stage（χ2=16.712,P0.05）,lymph node metastasis（χ2=6.381,P0.05）and depth of invasion（χ2=6.381,P 0.05）;but had nothing to do with the pathological grade（χ2=0.229,P0.05）or whether menopause（χ2= 0.004,P0.05）.Conclusion The positive expression rate of CK20 mRNA is increased significantly in endometrial cancer patients compared with benign uterine disease patients and healthy female volunteers,and it is related to the clinical stage,lymph node metastasis and depth of invasion of endometrial cancer;the specificity of CK20could be used as blood-borne micrometastases reference of endometrial cancer.

关 键 词：实时荧光定量PCR 外周血细胞 CK20基因 子宫内膜肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]