啤酒花黄酮的纯化工艺及其工艺参数的优化  

Purification process of total flavonoids from hops and its optimization of process parameters

作　　者：任治兴[1] 王玲[1] 潘志勇[1] 孙新[2] 卢日峰[1] 

机构地区：[1]吉林大学公共卫生学院卫生毒理学教研室,吉林长春130021 [2]吉林省人民医院营养科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2013年第5期1031-1035,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30873371);吉林大学基本科研业务费资助课题(2011)

摘　　要：目的:通过乙醇浸提和大孔树脂吸附的方式对啤酒花黄酮进行分离纯化,并根据黄酮的含量和收率优化工艺参数,建立适合规模化生产的啤酒花黄酮制备工艺。方法:在乙醇浸提啤酒花黄酮的基础上,采用大孔树脂静态吸附和洗脱方法分离纯化啤酒花黄酮。依据浸提效果、吸附率和洗脱率确定最佳工艺参数。结果:最佳工艺条件体积分数为40%乙醇浸提,采用FL-3大孔树脂,以pH值为4、20%乙醇条件下静态吸附,再以pH值为7、60%乙醇溶液洗脱。按此工艺制备的样品中黄酮的含量为70.6%,收率为70.2%。结论:乙醇浸提结合FL-3大孔树脂静态吸附方法可分离纯化啤酒花黄酮;该工艺操作简便,样品处理量大,适合规模化生产。Objective To isolate and purify hops flavonoids by ethanol extraction and macroporous resin adsorption,and to optimize process parameters according to content and yield of flavonoids,and to establish process system for large-scale production of hops flavonoids.Methods In the ethanol extraction based on the total flavonoids from Humulus lupulus,by static adsorption and elution method of macroporous resin the total flavonoids of hops were separated and purified.According to leaching effect and the rates of adsorption and elution,the optimal process parameters were determined.Results The optimal procedure was established,and the ethanol concentration was 40%;FL-3macroporous resin was applied on the condition that ethanol concentration was 20% and pH value of leacheate was 4with the absorption and elution experiment of static state,then the condition was changes,the condition of ethanol concentration 60%and pH value of leacheate 7was used and eluted.The recovery rate of total flavonoids from hops was 70.2%,and the purity quotient was 70.6%.Conclusion Ethanol extraction method combined with FL-3macroporous resin adsorption can achieve the purification of flavonoids from hops.This process has the advantages of simple operation and a large amount of sample processing,and it is suitable for large scale production.

关 键 词：啤酒花 黄酮 纯化 大孔树脂 静态吸附 

分 类 号：R284.2[医药卫生—中药学]