亚硒酸钠对不同年龄大鼠脊髓损伤后睫状神经营养因子及其受体的影响  

EFFECT OF SODIUM SELENITE ON CILIARY NEUROTROPIC FACTOR AND ITS RECEPTOR IN DIFFERENT-AGED RATS SUFFERING SPINAL CORD INJURY

作　　者：陈显兵[1,2] 胡玉萍[2] 王凤杰[1] 王丽芳[1] 谭文波[1] 袁德培[2] 

机构地区：[1]湖北民族学院医学院 [2]湖北民族学院中医药学院,恩施445000

出　　处：《营养学报》2013年第5期455-459,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.81260521/H2704);湖北省教育厅重点项目(No.D20131904)

摘　　要：目的探讨睫状神经营养因子(CNTF)及其受体(CNTFRа)在脊髓损伤中的变化及亚硒酸钠对脊髓损伤的保护作用。方法将不同年龄大鼠(幼年、成年、老年)随机分为对照组、模型组及亚硒酸钠处理组,阻断腹主动脉建立脊髓损伤模型,对照组不夹闭血管,亚硒酸钠处理组于术前2 w开始补充营养剂量的亚硒酸钠并夹闭腹主动建立脊髓损伤模型。分别于伤后1d、7d、14d处死动物,取脊髓分别作HE染色、免疫组化及RT-PCR检测,观察神经细胞结构及CNTF、CNTFRа的变化。结果正常大鼠各年龄组均有CNTF、CNTFRа蛋白及mRNA表达,幼年组强于成年组及老年组(P<0.05);亚硒酸钠干预组CNTF mRNA、CNTFRa mRNA表达较模型组增强,增强持续时间幼年组最长。结论睫状神经营养因子对大鼠脊髓损伤有保护作用,亚硒酸钠能上调CNTF、CNTFRа的表达。Objective To investigate the alteration of ciliary neurotrophic factor （CNTF） and its receptor （CNTFRa） after spinal cord injury and the protective effect of sodium selenite. Methods The different-aged rats were randomly divided into control group, experimental group and sodium selenite treated group. In experimental group, the abdominal aorta were ligated; In sodium selenite treated group, the rats were fed nutritional dosage of sodium selenite before ligating their abdominal aorta. After 1 d, 7 d or 14 d, the rats were killed to investigate the structure of nerver cells by HE staining and the expression of CNTF and CNTFRa by immunohistochemical method and RT-PCR. Results Both protein and mRNA of CNTF and CNTFRa were positively expressed in different-aged rats of control group. The mRNA levels of CNTF and CNTFRa in sodium selenite treated group were higher than experimental group. Conclusion Sodium selenite can up-regulate the expression of CNTF and CNTFRa. Ciliary neurotrophic factor plays protective roles agaisat the development of spinal cord injury.

关 键 词：脊髓损伤 睫状神经营养因子 亚硒酸钠 

分 类 号：R651.2[医药卫生—外科学]