番茄红素对大鼠急性心肌梗死所致心肌损伤的保护作用  

PROTECTIVE EFFECTS OF LYCOPENE ON ACUTE MYOCARDIAL INFARCTION IN RATS

作　　者：杨艳晖[1] 王海霞[1] 荣胜忠[1] 张广滕[1] 潘洪志[1] 

机构地区：[1]哈尔滨医科大学附属第二医学院,哈尔滨150086

出　　处：《营养学报》2013年第5期471-474,共4页Acta Nutrimenta Sinica

基　　金：黑龙江省卫生厅资助项目(No.2011-052)

摘　　要：目的探讨番茄红素对大鼠急性心肌梗死所致心肌损伤的保护作用及抗氧化作用机制。方法 60只雄性Wistar大鼠随机分为对照组、番茄红素高、低剂量组(番茄红素10和5 mg/kg bw)三组。对照组灌喂橄榄油。4w后,结扎三组大鼠心脏左冠状动脉前降支,建立大鼠急性心肌梗死模型,记录心电图,心脏切片计算心肌梗死面积。测定血清中肌酸肌酶(CK)、肌酸肌酶同工酶(CK-MB)、乳酸脱氢酶(LDH)及血清与心肌组织匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及脂质过氧化产物丙二醛(MDA)含量。结果番茄红素可明显降低血清CK、LDH活性及MDA含量,提高SOD活性(P<0.05)。结论番茄红素对急性心肌梗死具有明显保护作用,其机制可能与其增强抗氧化酶活性,减少自由基对心肌的氧化损伤,稳定生物膜有关。Objective To observe the protective effects of lycopene on myocardial infarction in rats. and explore its antioxidant mechanism. Methods Sixty Wistar rats were divided into 3 groups randomly. The rats in the high-dose and low-dose groups were given 10 and 5 mg/kg bw lycopene respectively. The rats in the control group were given olive oil. Four weeks later, the left anterior descending coronary artery of rats was ligated to establish acute myocardial infarction （AMI） model. The electrocardiogram （ECG） was analysed serum activity of superoxide dismutase （SOD）and glutathione peroxidase （GSH-Px）, and contents of malondialdehyde （MDA）, lactate dehydrogenase （LDH）, creatine kinase （CK）, isoenzyme of creatine kinase（CKMB） and infarct area were measured. Results Lycopene decreased infarct area of myocardium at the doses of 5 and 10mg/kg bw. Lycopene increased SOD activity, reduced serum MDA level. Conclusion Lycopene can protect rats against AMI through enhanced activities of SOD and myocardial cellular membrance stability.

关 键 词：急性心肌梗死 番茄红素 氧化应激 大鼠 

分 类 号：R542.22[医药卫生—心血管疾病]