枸杞多糖对体外人前列腺癌DU-145细胞增殖和凋亡的影响  

EFFECTS OF LYCIUM BARBARUM POLYSACCHARIDE ON PROLIFERATION AND APOPTOSIS OF HUMAN PROSTATE CARCINOMA DU-145 CELLS IN VITRO

作　　者：向春燕[1] 罗琼[1] 李菁菁[1] 赵颀涵[1] 阎俊[1] 李卓能[2] 

机构地区：[1]武汉大学公共卫生学院营养与食品卫生学系,武汉430071 [2]武汉市疾病预防控制中心,武汉430000

出　　处：《营养学报》2013年第5期489-491,495,共4页Acta Nutrimenta Sinica

基　　金：达能膳食营养研究与宣教基金(No.200305)

摘　　要：目的研究枸杞多糖(LBP)对人前列腺癌DU-145细胞的影响及其可能的机制。方法采用MTT法检测细胞抑制效应,原位末端标记法(TUNEL)检测细胞凋亡情况,流式细胞术检测细胞凋亡分布峰的变化,免疫组化法和Western-blot检测细胞Bcl-2、Bax蛋白的表达。结果 LBP可显著抑制DU-145细胞增殖;LBP诱导DU-145细胞的凋亡,与对照组相比,差异有统计学意义;流式细胞仪分析出现明显的凋亡峰,同时,DU-145细胞表达Bcl-2/Bax蛋白比值明显下降。以上各指标中,LBP的作用随着浓度增加而加强,呈现一定的剂量-反应关系。结论 LBP对人前列腺癌DU-145细胞增殖有抑制作用,其机制可能与其损伤人前列腺癌DU-145细胞DNA链、诱导细胞凋亡和调控凋亡相关基因表达有关。Objective To investigate the effects ofLycium barbarum polysaccharides （LBP） on the growth of human prostate carcinoma DU-145 cells and its mechanism. Methods The MTT assay was used to examine the inhibition effect of LBP on DU-145 cells, TUNEL was used to examine the apoptosis of DU-145 cells, and flow cytometry to analyze the DU-145 apoptosis. The immunohistochemical technique and Western blot method were used to detect Bcl-2 and Bax expression. Results LBP could inhibit the proliferation of DU-145 cells, induce DU-145 cell apoptosis and DNA damage. Compared with the control, there was significant difference and the chart of FCM showed obvious apoptosis peak. The ratio of Bcl-2/Bax protein was decreased significantly. Conclusion LBP could inhibit DU-145 cell line in vitro and the mechanism may be related to its influence on apoptosis, DNA damage, and Bcl-2/Bax expression.

关 键 词：枸杞多糖 人前列腺癌细胞 凋亡 基因表达 体外 

分 类 号：R737.25[医药卫生—肿瘤]