沙苑子黄酮类化合物的响应面法优化提取及其清除DPPH自由基能力  

Optimization of Extraction Conditions of Flavones from Semen Astragali Complanati by Response Surface Methodology and Its Scavenging Ability on DPPH Free Radical

作　　者：张清安[1] 范学辉[2] 刘梅[1] 张志琪[3] 

机构地区：[1]陕西师范大学食品工程与营养科学学院 [2]陕西师范大学体育学院 [3]陕西师范大学化学化工学院,西安710062

出　　处：《天然产物研究与开发》2013年第10期1339-1345,共7页Natural Product Research and Development

基　　金：国家自然科学基金项目(31101324);陕西省自然科学基金项目(2011JQ2003);中央高校基本科研业务费专项资金项目(GK201002014;GK201302039)

摘　　要：为了获得沙苑子黄酮类化合物的有效提取工艺参数,在单因素试验基础上选取液料比、提取温度、沙苑子粒度、提取时间作为试验因素,以提取物的吸光度为响应值,采用响应面法对提取工艺参数进行优化,并对优化条件下的提取物进行清除DPPH自由基能力研究。结果表明:60%甲醇是有效的提取剂;最佳工艺参数为粒度120目,温度42℃,提取时间47 min,液料比30∶1,该条件下沙苑子黄酮类物质的粗提率为10.66%;提取物具有一定的清除DPPH自由基效果,低浓度条件下呈现良好的量效关系,浓度为2%时清除率最高。In order to obtain the optimum extraction conditions for flavoness from Semen Astragali Complanati,on the basis of one-factor experiment,the ratio of solvent-to-sample,extraction temperature,particle size and extraction time were optimized by Response Surface Methodology （RSM） and the absorption of extracts was chosen as the response value.The optimized extraction conditions were determined as follows：the suitable extractant was 60％ methanol;the particle size was 120 mesh;the extraction temperature was 42 ℃ ;the extraction time was 47 min;the ratio of solvent-to-sample was 30：1.Under these conditions,the crude extraction yield of flavone was 10.66％.In addition,the DPPH scavenging ability of the extract was investigated.The results indicated that there was a good correlation between extract＇s concentration and scavenging rate at lower concentrations,and the highest DPPH scavenging ability was obtained when the concentration of flavone was 2％.

关 键 词：沙苑子 黄酮 响应面法 提取 自由基 

分 类 号：TS201.1[轻工技术与工程—食品科学]