响应曲面法优化虫草素和虫草多糖的综合提取工艺  

Optimization of Intergrated Extraction of Cordycepin and Polysaccharides by Response Surface Methodology

作　　者：邓黎[1] 王晓虹[1] 韩涛[1] 皮立[1] 韩发[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810008

出　　处：《天然产物研究与开发》2013年第10期1428-1435,1427,共9页Natural Product Research and Development

基　　金：中国科学院院地合作项目(Y12B031211);科技部人员服务企业行动项目(2009GJG20004);中国科学院科技支青项目(082B371211);国家自然科学基金项目(31070475)

摘　　要：以冬虫夏草和人工蛹虫草为试材,优化虫草素和虫草多糖的综合提取工艺。在提取方法比较试验以及超声波水提单因素试验基础上,利用响应曲面法考察超声提取时间、温度和液固比对虫草素和虫草多糖综合提取率的影响,优化提取工艺。得到最优提取工艺条件为:提取温度72℃,液固比30 mL/g,提取时间77 min,提取2次,虫草素提取率的理论值可达53.27%,虫草多糖提取率的理论值可达41.92%。This paper was aiming to optimize the extraction process for extracting Cordycepin and polysaccharides from Cordyceps sinensis and cultured Cordyceps militaris.On the basis of single-factor experiments,three important extraction factors,namely ultrasonic extraction time,temperature and the ratio of material to liquid were optimized using response surface methodology.The results showed that the optimal extraction conditions of cordycepin and polysaccharides were：72 ℃ as extraction temperature,30 mL/g as the ratio material to liquid,77min as extraction time,and extracted for two times.The theoretical extraction rates of cordycepin and polysaccharides were up to 53.27％ and 41.92％,respectively.

关 键 词：虫草素 虫草多糖 冬虫夏草 蛹虫草 响应曲面法 

分 类 号：S567.3[农业科学—中草药栽培]