脑胶质瘤中Rad 51及端粒酶逆转录酶的表达研究  

Study of Rad 51 and hTERT expression in gliomas

作　　者：王奕丹[1] 章建国[2] 田薇[2] 黄华[2] 王东林[3] 

机构地区：[1]南通大学公共卫生学院预防医学专业092班,江苏南通226001 [2]南通大学附属医院病理科 [3]南通大学医学院病理学系

出　　处：《现代预防医学》2013年第21期4015-4018,共4页Modern Preventive Medicine

基　　金：国家自然科学基金项目(81272789);江苏省高等学校大学生实践创新训练计划项目(2011856);南通市科技局应用研究项目(BK2012065)

摘　　要：目的探讨Rad51及端粒酶逆转录酶在胶质瘤中的表达及与预后的关系。方法应用免疫组织化学SP法检测106例胶质瘤中Rad51及hTERT的表达。结果 (1)Rad51在胶质瘤中表达的总阳性率为52.8%(56/106),Ⅱ、Ⅲ、Ⅳ级的阳性率分别为31.3%(10/32)、55.9%(33/59)、86.7%(13/15);(2)hTERT在胶质瘤中表达的总阳性率为54.7%(58/106),Ⅱ、Ⅲ、Ⅳ级的阳性率分别为37.5(12/32)、57.6%(34/59)、80.0%(12/15);(3)Rad51蛋白表达和hTERT表达呈正相关(r=0.5428,P﹤0.01);(4)Ⅱ、Ⅲ、Ⅳ级胶质瘤患者术后平均生存时间分别为32.8、20.1、10.7个月;Rad51和hTERT阴性表达与阳性表达者间患者平均生存时间的差异均有统计学意义(P﹤0.05)。结论 Rad51和hTERT的表达与胶质瘤的病理分级呈正相关,与患者的术后生存时间呈负相关,两者联合检测有助于胶质瘤的早期诊断及预后评估。OBJECTIVE To investigate the expressions of Rad51 and hTERT in gliomas and discuss their relationship to the prognosis. METHODS SP immunohistochemistry was used to detect expressions of Rad51 and hTERT in 106 human gliomas. RESULTS （1） The positive rate of Rad51 in gliomas was 52.8% （56/106）. In Ⅱ , Ⅲ, Ⅳ grade of gliomas, the positive rates of Rad51 was 31.3% （10/32）, 55.9 % （33/59） and 86.7% （13/15）, respectively. （2） The positive rate of hTERT in gliomas was 54.7% （58/106）. In Ⅱ , Ⅲ, Ⅳ grade of gliomas, the positive rates of hTERT was 37.5% （12/32）, 57.6% （34/59） and 80.0% （12/15）, respectively. （3） Expressions of Rad51 and hTERT were positive relationship （r = 0.5428, P〈 0.01）. （4） The average living time of Ⅱ , Ⅲ, Ⅳ grade of gliomas after operations was 32.8, 20.1, 10.7 months, respectively. A statistically significant difference were observed in the patients＇average living time between negative and positive expression of Rad51 and hTERT （P 〈 0.05）. CONCLUSION The expressions of Rad51 and hTERT are positively correlated to the grades of glioma and negatively correlated to the survival time, Combined assay could be helpful of early diagnosis and prognosis evaluation.

关 键 词：胶质瘤 RAD51 人端粒酶逆转录酶 预后 

分 类 号：R739.41[医药卫生—肿瘤]