干旱胁迫对金心吊兰叶片活性氧及其清除系统的影响  

Effects of drought stress on reactive oxygen species and their scavenging systems in Chlorophytum capense var.medio-pictumleaf

作　　者：贾学静[1] 董立花 丁春邦[1] 李旭[1] 袁明[1] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《草业学报》2013年第5期248-255,共8页Acta Prataculturae Sinica

基　　金：四川省教育厅青年基金项目(11ZB054)资助

摘　　要：以金心吊兰叶片边缘的绿色区域与中心黄白色区域为材料,研究了干旱胁迫对其活性氧及其清除系统的影响,结果表明,1)随着胁迫时间的延长,叶片各区域的过氧化氢(H2O2)、超氧阴离子(O2-·)含量和丙二醛(MDA)含量均表现出逐渐上升的趋势,而超氧化物歧化酶(SOD)与过氧化物酶(POD)活性则先上升后下降。2)随着胁迫浓度的增加,叶片中活性氧(H2O2,O2-·)含量、抗氧化酶(SOD,POD)活性和MDA含量表现出上升的趋势。3)采用隶属函数法对吊兰叶片的抗旱性进行了综合评价,其抗旱性表现出位置效应,叶片中心区域抗旱性强于边缘区域。4)吊兰叶片的抗旱性与叶绿素含量呈负相关,叶绿素含量相对高的区域其抗旱性相对较低,金心吊兰抗旱性强于野生型。The effects of drought stress on reactive oxygen species and their scavenging systems of leaf blade and the center parts of Chlorophytum capense var. medio-pictum were studied. 1） The content of H2 O2, O2 - and MDA gradually increased with the stress time, while the activity of SOD and POD increased in the earlier days, but then declined. 2） The content of reactive oxygen species （H2O2 and O2- ）, activity of scavenging enzymes （SOD and POD） and the content of MDA increased gradually as the PEG concentration increased. 3） The com- prehensive evaluation of drought tolerance by the membership function method indicated that there were clear position effects, and drought resistance in the center part of the leaf was stronger than that in the blade of the leaf. 4） Drought resistance was negatively correlated with chlorophyll content： the higher the chlorophyll con- tent, the weaker the drought tolerance. Drought resistance in C. capense var. medio-pictum was better than that of C. capense.

关 键 词：金心吊兰 干旱胁迫 活性氧 丙二醛 保护酶系统 

分 类 号：S682.36[农业科学—观赏园艺]