下咽鳞状细胞癌组织中ΔNp63和TAp63蛋白的表达及临床意义  

Expression and clinical significance of ΔNp63 and TAp63 protein in hypopharyneal squamous cell carcinoma

作　　者：林树春[1] 林国经[1] 黄建民[1] 

机构地区：[1]福建医科大学附属协和医院耳鼻咽喉科,福州350001

出　　处：《中国肿瘤临床与康复》2013年第10期1061-1065,F0003,共6页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的探讨下咽鳞状细胞癌(HSCC)和下咽乳头状瘤及正常下咽黏膜中ΔNp63和TAp63蛋白的表达及其在下咽鳞状细胞癌发病机制中的作用。方法采用免疫组织化学SP法检测34例下咽鳞状细胞癌、5例下咽乳头状瘤及8例下咽正常黏膜组织中ΔNp63和TAp63蛋白的表达情况。结果 (1)ΔNp63蛋白在下咽正常黏膜、乳头状瘤及下咽癌组织中的阳性细胞百分数为(21.6±12.5)%,(43.6±10.2)%和(75.3±24.5)%,其阳性细胞百分数越高,组织分化程度越低(P<0.05),但阳性细胞百分数的高低与TNM分期、淋巴结转移无关(均P>0.05);(2)TAp63蛋白在下咽正常黏膜、乳头状瘤及下咽癌组织中的阳性表达率为(14.5±10.2)%,(13.6±5.2)%和(6.7±5.2)%,其阳性细胞百分数越低,组织分化程度越低(P<0.05),但阳性细胞百分数的高低与TNM分期、淋巴结转移无关(均P>0.05)。结论 p63的异常表达可能参与了下咽鳞状细胞癌发生的早期阶段,ΔNp63的过度表达及TAp63的表达下调在下咽鳞状细胞癌的形成机制中有重要作用,并与下咽癌的分化程度相关,但与临床分期及淋巴结转移无关。Objective To investigate the expression of △Np63 and TAp63 protein in hypopharyn- geal squamous cell carcinoma（ HSCC ）, papillary epithelioma（ HPE ） and normal mucosa（ HNM ） and the role of △Np63 and TAp63 gene aberration may be involved in HSCC. Methods The △Np63 and TAp63 protein expression was detected by immunohistochemistry in 34 cases of HSCC, 5 cases of HPE, 8 cases of HNM . Results （ 1 ） The mean proportion of positive cells of △Np63 protein in HNM, HPE, HSCC was （21.6±12.5）%,（43.6±10.2）%,（75.3±24.5）% respectively, and increased withpoorer differentiation, but no correlation was found with TNM stage and lymph node metastasis （ P 〉 0. 05 ） . （ 2 ） The mean proportion of positive cells of TAp63 protein in HNM, HPE, HSCC was （14.5 ±10. 2）% , （13.6 ± 5.2） % , （ 6. 7 ± 5.2 ） % respectively, and decreased with poorer differentiation, but no correlation was found with TNM stage and lymph node metastasis too （ P 〉 0. 05 ） . Conclusions Abnormal expression of p63 may be involved in he early phases of hypopharyngeal tumorigenesis . △Np63 protein over-expression and TAp63 protein down-regulation may play a pivotal role in hypopharyngeal tumorigenesis, and is correlated with the differentiation, but is not correlated with clinical stage and lymph node metastasis in HSCCs.

关 键 词：下咽肿瘤 P63蛋白 免疫组织化学 

分 类 号：R739.63[医药卫生—肿瘤]