卵巢上皮癌组织中活性氧与乏氧诱导因子-1α的表达及其相关性  

Expression of reactive oxygen species and hypoxia inducible factor-1α in epithelial ovarian cancer and their interaction

作　　者：张艳[1] 靳卫国[1] 李爱华[1] 

机构地区：[1]山东省聊城市人民医院妇科,252000

出　　处：《肿瘤研究与临床》2013年第10期663-668,共6页Cancer Research and Clinic

摘　　要：目的 检测卵巢上皮癌组织中乏氧诱导因子-1α（HIF-1α）mRNA、蛋白以及活性氧（ROS）的表达,探讨卵巢上皮癌组织中氧代谢的异常,以及两者在卵巢上皮癌发生发展中的相互作用.方法 应用实时RT-PCR法和免疫组织化学Elivision plus二步法分别测定卵巢上皮癌组织中HIF-1αmRNA及蛋白的表达；应用DCFH-DA法检测组织中ROS的表达.结果 卵巢上皮癌组织HIF-1 αmRNA相对含量（4.466±0.754）明显高于良性卵巢肿瘤（6.073±1.053）（t=-7.701,P=0.000）,良性卵巢肿瘤、早期（5.048±0.645）及晚期卵巢上皮癌（4.146 ±0.632）HIF-1α mRNA表达呈递增趋势（t=5.301,P=0.000）；HIF-1α mRNA表达与组织学分级及淋巴结转移呈正相关（t=3.724、-3.851,均P=0.000）.HIF-1 α蛋白在卵巢上皮癌组织中阳性表达率[63.49％（40/63）1明显高于良性对照[36.36％（8/22）]（U=433,P=0.005）,HIF-1 α蛋白阳性表达率与淋巴结转移及临床分期呈正相关（x2=4.289、12.360,P=0.038、0.000）.卵巢上皮癌组织中ROS表达水平为110.239±18.414,较良性对照（58.366±20.360）明显升高（t=11.068,P=0.000）；ROS表达与卵巢上皮癌组织学分级、淋巴结转移及临床分期呈正相关（t=-2.055、2.816、-4.138,P=0.044、0.007、0.000）.在卵巢上皮癌组织中HIF-1 α蛋白表达与ROS呈中度正相关（r=0.572,P=0.000）；在中低分化组及晚期组中两者呈中度正相关（r=0.540,P=O.002;r=0.572,P=0.000）；在淋巴结转移组中两者呈高度正相关（r=0.703,P=0.001）；HIF-1α mRNA与ROS表达呈低度相关（r=0.376,P=0.002）.结论 乏氧是卵巢上皮癌的基本特征,随着肿瘤进展乏氧程度逐渐加重；HIF-1α的异常表达与卵巢上皮癌潜在的恶性生物学行为密切相关,可能影响卵巢上皮癌预后.在乏氧状态下,ROS与HIF-1 α可能参与肿瘤的发生过程.在卵巢上皮癌侵袭转移过程中,ROS对HIF-1α的正向调节可能发挥重要作用.肿瘤乏氧状态下,ROS�Objective To detect the expression of hypoxia inducible factor-lα （HIF-1α） in both mRNA and protein and reactive oxygen species （ROS） levels in human epithelial ovarian cancer,and investigate the abnormal state of oxygen metabolism and their interaction in human epithelial ovarian cancer develoment.Methods The expression of HIF-1α in mRNA and protein in epithelial ovarian cancer were detected by real time reverse transcription-polymerase chain reaction and immunohistochemical Elivision plus two-step method,and the level of ROS was detected by fluorescence microplate reader.Results The relative content of HIF-1α mRNA in the epithelial ovarian cancer group was 4.466±0.754,which was higher than that of the benign control group （6.073±1.053） （t =-7.701,P =0.000）.The expression of HIF-1α mRNA was raised gradually in tissues of the benign ovarian tumor,early and advanced stage of epithelial ovarian cancer （t =5.301,P =0.000）.The expression of HIF-1α mRNA in the epithelial ovarian cancer had positive correlation with grade and lymph node metastasis （t =3.724,-3.851,P =0.000）.The positive expression rate of HIF-1 α protein in epithelial ovarian cancer was 63.49 % （40/63）,which was higher than that of the benign group （U =433,P =0.005）,which had positive correlation with lymph node metastasis and stage （x2 =4.289,12.360; P =0.038,0.000）.The expression of ROS in the epithelial ovarian cancer was 110.239±18.414,which was higher than that of the benign group （58.366±20.360） （t =11.068,P =0.000）,which had positive correlation with grade,lymph node metastasis and stage （t =-2.055,2.816,-4.138; P =0.044,0.007,0.000）.There was a moderate positive correlation between the expression of HIF-1α mRNA and ROS in epithelial ovarian cancer （r =0.572,P =0.000）,and that was similar in low differentiation group and advanced group,there was a relatively high positive correlation in lymph node metastasis group （r =0.703,P =0.001）,there was a relatively low correlation betwee

关 键 词：卵巢上皮癌 活性氧 乏氧诱导因子-1Α 氧代谢 

分 类 号：R737.31[医药卫生—肿瘤]