5-氮杂-2'-脱氧胞苷体外对黑素瘤细胞系肿瘤相关抗原和免疫分子表达的影响  

Effect of 5-aza-2'-deoxycytidine on expressions of tumor-associated antigens and immune molecules in melanoma cell lines in vitro

作　　者：柴丽娜[1] 王捷熙[2] 李伟静[2] 刘敏霞 王璇琳[2] 王艳[2] 贺敏[2] 杨超[2] 于群[2] 韩颖[2] 任素萍[2] Adam I.Riker 

机构地区：[1]中南大学湘雅医学院基础医学院,长沙410083 [2]军事医学科学院野战输血研究所,北京100850 [3]Advocate Cancer Institute,Advocate Medical Group,Illinois 60453,USA

出　　处：《军事医学》2013年第10期740-744,共5页Military Medical Sciences

基　　金：国家自然科学基金资助项目(81071709)

摘　　要：目的探讨5-氮杂-2'-脱氧胞苷(5-aza-2'-deoxycytidine,5-aza-CdR)对4种体外培养建系的黑素瘤细胞肿瘤相关抗原和免疫分子表达水平的影响。方法将手术切除或细针穿刺活组织检查获得的黑素瘤组织体外培养建系;应用流式细胞术检测5-aza-CdR用药后黑素瘤细胞表面人类白细胞抗原(human leukocyte antigen,HLA)-Ⅰ类、-Ⅱ类、-A2以及细胞间黏附分子1(intercellular adhesion molecule 1,ICAM-1)表达的变化;采用实时定量RT-PCR检测用药后一系列重要的肿瘤相关抗原表达的变化。结果 5-aza-CdR用药后,4种黑素瘤细胞系HLA-Ⅰ类、-Ⅱ类和-A2的表达与对照组相比无明显变化;TC12A和TC13细胞ICAM-1表达明显升高;TC12A、TC13和TC69B细胞肿瘤相关抗原黑素瘤抗原基因(MAGE)-4和NY-ESO-1的表达水平明显升高。结论 5-aza-CdR不影响黑素瘤细胞HLA的表达水平,对ICAM-1和某些黑素瘤相关肿瘤抗原的表达有明显上调作用,提示化疗药物5-aza-CdR不会阻碍抗肿瘤免疫中黑素瘤细胞的抗原呈递能力,为化疗联合免疫治疗肿瘤提供了理论前提。Objective To explore the expression levels of tumor-associated antigens and immune molecules in four melanoma cell lines treated with 5-aza-2＇-deoxycytidine（5-aza-CdR） in vitro. Methods Melanoma cell lines were derived from tumor samples obtained by surgical excision or fine needle aspiration. Expressions of human leukocyte antigen （HLA） - I,- [I and -A2 and intercellular adhesion molecule 1 （ ICAM-1 ） were examined by flow cytometry. Expression changes of a series of tumor-associated antigens were performed by real-time quantitative RT-PCR. Results After 5-aza-CdR treatment, the expression level of HLA- I,- ]I and -A2 molecules in four melanoma cell lines did not change significantly compared with the control group, unlike ICAM-1 molecules in TC12A and TCI3 cells. Meanwhile, the expression of MAGE-4 and NY-ESO-1 in TC12A, TC13 and TC69B cells was up-regulated after 5-aza-CdR treatment. Conclusion 5-Aza-CdR up- regulates ICAM-1 and some melanoma-associated antigens without affecting the expression level of HLA antigens, suggesting that the chemotherapy drug 5-aza-CdR does not hinder the antigen presentation of melanoma cells in antitumor immune re- sponses.

关 键 词：黑色素瘤 5-氮杂-2’-脱氧胞苷 HLA抗原 ICAM-1 肿瘤相关抗原 

分 类 号：R739.5[医药卫生—肿瘤]