GPR37在骨髓瘤细胞黏附介导的耐药中的作用及意义  

The role and significance of GPR37 in myeloma cell adhesion mediated drug resistance

作　　者：王燏婵[1] 黄娴婷[1] 

机构地区：[1]南通大学医学院病原生物学系,南通226001

出　　处：《南通大学学报（医学版）》2013年第5期347-349,共3页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学青年基金资助项目(81201858);江苏省自然科学青年基金资助项目(BK2012231)

摘　　要：目的:研究G蛋白耦联受体37(orphan G protein-coupled receptor 37,GPR37)在细胞黏附介导的多发性骨髓瘤细胞耐药过程中的表达变化及其生物学作用。方法:采用多发性骨髓瘤细胞株RPMI 8226与纤黏蛋白(fibronectin,FN)或骨髓基质细胞株HS-5共培养构建细胞黏附模型。Western Blot检测GPR37分别在悬浮和黏附状态的RPMI 8226细胞中的蛋白表达水平。钙黄绿素实验检测改变GPR37的表达对RPMI 8226细胞黏附的影响,并采用化疗药物多柔比星处理细胞,CCK-8试剂盒检测上述处理对RPMI 8226细胞活力的影响。结果:Western Blot结果显示GPR37在RPMI8226细胞黏附模型中低表达。钙黄绿素实验结果显示RPMI 8226细胞过表达GPR37后其黏附能力显著降低。CCK-8实验结果表明GPR37过表达能显著增强RPMI 8226细胞对化疗药物多柔比星的敏感性。结论:GPR37可能通过影响骨髓瘤细胞与基质细胞的黏附能力从而影响其对化疗药物的敏感性。Objective： To investigate the expression and role of orphan G protein-coupled receptor 37 in myeloma cell adhesion mediated drug resistance. Methods： The model of bone marrow microenvironment was constructed by eocuhuring RPMI 8226 cells with bone marrow storma cells. Western Blot was used to determine the expression of GPR37 in suspended or adhesioned RPMI 8226 cells. Calcein-AM was used to detect the adhesion rate of RPMI 8226 cells. And we also used CCK-8 kit test the cell viability after treatment with Doxorubicin. Results： Western Blot analysis showed the expression of GPR37 was lower in adhesioned RPMI 8226 cells. When cells were overexpressed with GPR37, the quantity of adherent cell was reduced and GPR37 overexpression could lead to the enhanced sensitivity of RPMI 8226 cells treated with Doxorubicin. Conclusion： These datas revealed that GPR37 may play a role in myeloma cell adhesion which would mediate drug resistance.

关 键 词：骨髓瘤 黏附 耐药 G蛋白耦联受体37 

分 类 号：R733.3[医药卫生—肿瘤]