反相高效液相色谱法测定川芎饮片、实验大鼠血清和脑匀浆中的阿魏酸  

Determination of Ferulic Acid in Chuanxiong and in Animal Serum and Cerebrospinal Fluid by Reversed-Phase High Performance Liquid Chromatography

作　　者：吕琨[1] 丁明玉[1] 李红霞[1] 刘德麟[2] 

机构地区：[1]清华大学化学系,北京100084 [2]中国中医研究院基础理论所,北京100700

出　　处：《色谱》2000年第6期518-520,共3页Chinese Journal of Chromatography

基　　金：国家中医药管理局科研基金! (97Z0 6 7);清华大学 985重点项目资助

摘　　要：用反相高效液相色谱法测定了川芎饮片、实验大鼠血清和脑匀浆中的阿魏酸。分离柱为Nova Pak C18(3 9mmi.d .× 15 0mm) ,流动相为甲醇 水 乙酸 (体积比为 35∶6 5∶0 5 ) ,在 32 0nm波长下检测 ,用外标法定量。阿魏酸在 0 85mg/L～ 4 0 0mg/L范围内有良好的线性关系 ,线性相关系数为0 9990 4。阿魏酸的加标回收率为 95 %～ 10 2 %。An easy, rapid and sensitive method for the determination of ferulic acid(FA) in Chuanxiong extracts, animal (mouse) serum and cerebrospinal fluid by RP HPLC has been developed The FA was separated on an ODS column, Nova Pak  C 18 (3 9 mm i.d.×150 mm) and detected at the wavelength of 320 nm The mobile phase was methanol water acetic acid (35∶65∶0 5, V/V ),with a flow rate of 0 8 mL/min The detection limit of FA was 1 7 μg/L( S/N =3) and the calibration curve was linear within the range of 0 85 mg/L 4 00 mg/L (r=0 999 04,n=6) The mean recovery from animal serum and cerebrospinal was 95% 102%

关 键 词：阿魏酸 川芎 血清 脑匀浆 RP-HPLC 测定 中药 

分 类 号：R282.71[医药卫生—中药学] R284[医药卫生—中医学]