基于体外溶出度与抗肝纤维化作用效应动力学的冬虫夏草粉碎度研究  

Studies on grinding degree of Cordyceps sinensis based on in vitro dissolusion and pharmacodynamics of anti-hepatic fibrosis

作　　者：白金霞[1,2] 韩晋[2] 戴领[1,2] 徐和[1,2] 申宝德[2] 尹蓉莉[1] 袁海龙[2] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]中国人民解放军第302医院,北京100039

出　　处：《中草药》2013年第20期2823-2827,共5页Chinese Traditional and Herbal Drugs

基　　金：国家新药创制重大专项(2011ZX09201-201-14,2011ZX09201-201-35);国家自然科学基金资助项目(81073069)

摘　　要：目的从血清药理学的角度，结合体外溶出度实验，研究冬虫夏草用于抗肝纤维化最佳的粉碎度。方法冬虫夏草粉碎后分别过100、150、200、300目筛，制成不同粉碎度的冬虫夏草粉样品，结合腺苷的体外溶出度实验，采用体外肝纤维化细胞模型，不同粉碎度的冬虫夏草粉大鼠远给药后，以大鼠含药血清对HSC—T6细胞增殖的影响为指标，采用效应动力学方法研究不同粉碎度的冬虫夏草粉对肝纤维化细胞的抑制作用。结果粉碎度为200-300目冬虫夏草粉腺苷累积溶出度较好，冬虫夏草粉含药血清具有较好的抑制HSC-T6细胞增殖的作用，200-300目冬虫夏草粉含药血清对HSC-T6抑制的效应动力学曲线下面积较大。结论基于体外溶出度与效应动力学的方法可用于冬虫夏草治疗肝纤维化的粉碎度研究，粉碎度以200-300目最优。Objective To study the optimum particle size of Cordyceps sinensis for liver fibrosis in combination with in vitro dissolution experiment from serum pharmacology. Methods To prepare the powder samples with different grinding degrees, Cordyceps sinensis was crushed through 100-, 150-, 200-, and 300-mesh sieves. The in vitro dissolution of adenosine was measured at different time points. Meanwhile, the powder sample was ig administered to rats, and pharmacodynamic approach was adopted to study the inhibition of medicated serum on HSC-T6 proliferation. Results The accumulative in vitro dissolution ofC. sinensis by 200--300 meshes was higher than that of other meshes. Medicated serum could significantly inhibit HSC-T6 cell proliferation. The AUC of HSC-T6 inhibition kinetics of medicated serum crushed to 200--300 meshes was significantly higher than that in other groups. Conclusion The in vitro dissolution and pharmacodynamic method could be used for the study on different particle sizes of C. sinensis for anti-hepatic fibrosis, and 200--300 meshes are the optimal particle size.

关 键 词：冬虫夏草 粉碎度 腺苷 HSC—T6 效应动力学 肝纤维化 

分 类 号：R285.5[医药卫生—中药学]