唾液酸化Lewis X抗原DNA适配子体外抑制乳腺癌细胞侵袭转移  

DNA aptamer against sialyl lewis X inhibits metastasis of breast cancer cells in vitro

作　　者：阳芳 冯茂辉[2] 谢伟[2] 陈双倩[2] 王国洲[2] 陈大平[2] 杨倩[2] 方喜平[2] 柳琨[2] 

机构地区：[1]湖南省永州市中心医院肿瘤内科,425000 [2]武汉大学中南医院肿瘤外科肿瘤生物学行为湖北省重点实验室,430071

出　　处：《中华实验外科杂志》2013年第11期2295-2298,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81072152）;湖北省自然科学基金资助项目（2009CD201）;湖北省卫生厅科研资助项目（2013.JX6B20）;武汉市科技攻关重点资助项目（200860423230）

摘　　要：目的 观察特异结合唾液酸化Lewis X抗原（SLeX） DNA适配子抑制MDA-MB-231细胞与E-选择素黏附能力及其体外抑制MDA-MB-231细胞浸润转移能力.方法 采用体外细胞黏附实验、Transwell小室侵袭实验,检测E-选择素及唾液酸化Lewis X的DNA适配子对肿瘤细胞黏附力、侵袭力的影响.结果 该DNA适配子可以有效的抑制MDA-MB-231细胞与E-选择素黏附,对E-选择素处理过的MDA-MB-231,随着适配子浓度的增加,其抑制细胞黏附的作用越强,抑制率（％）分别为15.54 ±6.10、39.69±5.69、62.15±5.44；适配子组（200 μmol/L）吸光度（A）值与SLeX抗体（1∶100）组比较,差异有统计学意义（P＜0.05）,说明其抑制黏附作用较抗体强.E-选择素预先处理过的Transwell侵袭实验中,各适配子组（50、100、200 μmol/L）穿膜细胞数分别为（199.3±3.2）、（178.7±3.5）、（168.3±4.2）个（F=88.6,P＜0.05）；两两比较差异有统计学意义（P＜0.05）,说明随着SLeX适配子浓度的增高,抑制细胞侵袭的作用越强,抑制率依次为（25.13±5.05）％、（57.59±5.52）％、（73.83 ±6.54）％；适配子组（200 μmol/L）较SLeX抗体（1∶ 100）组穿膜细胞数少（P＜0.05）,说明适配子比抗体抑制侵袭作用强.结论 特异结合SLeX DNA适配子可以抑制MDA-MB-231细胞与E-选择素黏附,阻断Lewis-selectin途径,抑制MDA-MB-231细胞体外侵袭转移.Objective To study the effect of the sialyl lewis X （SLeX） binding DNA aptamer on adhesion and metastasis of breast cancer cells in MDA-MB-231 in vitro. Methods The capability of cancer cell adhesion was in vitro verified with methyl thiazol tetrazolium （MTY） assay. The invasion of cancer cells was tested by transwell assay. Results The DNA aptamers could inhibit adhesion of cancer cells in duced by E-selectin and SLeX. The inhibition ratio was 15.54%, 39.69% and 62. 15% when the concen trations of DNA aptamers were 50, 100 and 200 μmol/L, respectively. The inhibitory effect of DNA aptamers with 100 mol/L matched that of the antibodies （ 1 ： 100） , meanwhile, the inhibitory effect of DNA aptamers with 200 μmol/L was stronger than antibodies （ P 〈 0. 05 ）. The data revealed that DNA aptamer could inhibit cancer cell invasion. The suppression ratio was 25.13% , 57.59% and 73.83% when the concentrations of DNA aptamers was 50, 100 and 200 μmol/L respectively. The effect of the DNA aptamers with 200 μmol/L was stronger than the antibodies （ P 〈 0. 05 ）. Conclusion These results indicate that the SLeX binding DNA aptamer can effectively and significantly inhibit the adhesion, migra- tion and invasion in MDA-MB-231 cells in vitro.

关 键 词：唾液酸化LEWIS X抗原 DNA适配子 乳腺癌 侵袭 转移 

分 类 号：R737.9[医药卫生—肿瘤]