重组腺病毒载体pAdxsi-GFP-Galectin-1的构建及鉴定  

Construction and identification of the recombinant adenovirus vector pAdxsi-GFP-Galectin-1

作　　者：陈炜[1] 江从庆[1] 刘先桂[1] 张志远[1] 刘韦成[1] 丁召[1] 钱群[1] 刘志苏[1] 

机构地区：[1]武汉大学中南医院结直肠肛门外科湖北省肠病医学临床研究中心肠病湖北省重点实验室,430071

出　　处：《中华实验外科杂志》2013年第11期2315-2317,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81070387）

摘　　要：目的 构建及鉴定重组腺病毒载体pAdxsi-GFP-Galectin-1.方法 通过Kpn I/BamH I双酶切从原始质粒获取半乳糖凝集素-1完整基因片段并连接到pSbuttle-CMV-EGFP重组穿梭载体；通过I-Ceu I＋I-Sce I双酶切获取CMV-GFP-Galectin-1完整基因片段并连接到pAdxsi载体上,得到重组腺病毒载体pAdxsi-GFP-Galectin-1.结果 通过基因测序、酶切鉴定证实目的基因克隆至重组腺病毒载体,目的基因序列与Genebank录入序列一致.结论 成功构建重组腺病毒载体pAdxsi-GFP-Galectin-1.Objective To construct and identify the recombinant adenovirus vector pAdxsi-GFP-Galectin-1.Methods Whole sequence of galectin-1 was obtained from original plasmid double-digested with Kpn I/BamH I enzymes.Then Galectin-1 DNA segments were linked into pShuttle-CMV-EGFP vector to obtain recombinant pShuttle-GFP-Galectin-1 plasmid.Next,recombinant DNA sequences CMV-GFP-Galectin1were double-digested with I-Ceu I/I-Sce I enzymes and constructed into pShuttle-GFP-Galectin-1 vector to establish recombinant adenovirus vector pAdxsi-GFP-Galectin-1.Results Through endonuclease and gene sequencing the target gene was verified to be corrrctly cloned in adenvrius vector.Conclusion The recombinant adenovirus vector pAdxsi-GFP-Galectin-1 was successfully constructed.

关 键 词：重组腺病毒载体 GALECTIN-1 

分 类 号：R346[医药卫生—基础医学]