胃癌变患者c-myc mRNA与幽门螺杆菌感染及细胞程序性死亡的关系  

The c-myc mRNA and helicobacter pylori infection and the relationship between the programmed cell death in the with Gastric cancer in the patients with gastric cancer

作　　者：潘晟[1] 罗浩[1] 李力[1] 

机构地区：[1]武汉科技大学附属医院武汉市普仁医院胃肠外科,430000

出　　处：《中华实验外科杂志》2013年第11期2408-2410,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨不同胃病变患者的发生发展直至恶变过程中c-myc mRNA的表达与幽门螺杆菌（Hp）感染的相关性及其与胃组织细胞程序性死亡的关系.方法 通过实时荧光定量逆转录-聚合酶链反应（FQ-PCR）检测72例慢性浅表性胃炎（CSG）、55例慢性萎缩性胃炎（CAG）、52例肠化生（IM）、46例不典型增生（AH）、65例胃癌（GC）组织（分别为CSG、CAG、IM、AM、AH、GC组）中c-myc mRNA的表达；快速尿素酶法、Warthin-Starry银染法和甲苯胺蓝染色联合检测胃黏膜Hp感染；原位末端标记法（TUNEL）检测胃黏膜细胞程序性死亡指数（AI）.结果 CSG、CAG、IM、AM、AH、GC组胃黏膜组织中c-myc mRNA的阳性率分别为21.5％ （7/31）、31.0％（8/27）、41.0％（10/26）、59.1％ （14/24）、69.1（23/33）,c-myc mRNA相对表达水平分别为0.089±0.002、0.0113±0.014、0.168 ±0.028、0.226±0.040、0.367±0.037,IM、AH、GC组c-myc mRNA表达量均高于CSG、CAG组（P＜0.01）,AH组高于IM组（P＜0.01）,GC组高于IM、AH组（P＜0.01）.c-myc mRNA表达量与胃黏膜细胞AI呈负相关（L=-0.700,P＜0.05）；IM、AH、GC组Hp阳性患者胃黏膜细胞中c-myc mRNA的表达均高于Hp阴性患者（P＜0.05或0.01）.结论 胃黏膜癌变过程中,Hp感染能上调胃黏膜细胞中c-myc mRNA的表达,而抑制异形细胞程序性死亡而发挥致癌作用.Objective To discuss different stomach trouble change occurred in the process of de- velopment until the progression in patients with c-myc mRNA expression in correlation with helicobacter py- lori （Hp） infection and its relationship with gastric tissue programmed cell death, Methods By real-time fluorescent quantitative reverse transcription-olymerase chain reaction （FQ-PCR） to detect 72 cases of chronic superficial gastritis （CSG） ,55 cases of chronic atrophic gastritis （ the CAG） ,52 cases of intestinal metaplasia （IM） ,46 cases of atypical hyperplasia （AH） ,65 cases of gastric cancer （GC） group （CSG group,respectively,the CAG group, IM, AM group, AH, the GC group） in the c-myc mRNA expression; Rapid enzymatic urea, Warthin Starry silver staining and toluidine blue staining assay for gastric mucosa Hp infection;In situ end labeling （TUNEL） method to detect gastric mucosal cell apoptosis index （AI）. Re- suits CSG group,the CAG group,IM,AM group,AH,the GC group c-myc in gastric mucosa tissue mRNA positive example were 21.5% （7/31） ,31.0% （8/27） ,41.0% （10/26） ,59. 1% （14/24） and 69.1 （23/33） ,c-myc relative mRNA expression levels were 0. 089 ±0.002,0. 0113 ± 0. 014 ram,0. 168 ± 0. 028,0. 226,0. 367 ＋0. 037 ram,0. 040 mm IM,AH group,GC group c-myc amount of mRNA expression were higher than in CSG, the CAG group （ P 〈 0. O1 ）, the AH group is higher than the IM group （ P 〈0.01 ）, the GC group higher than IM, AH group （P 〈 0.01 ）. C-mye mRNA expression quantity and nega- tively correlated with gastric mucosa cells AI （rs =-0. 700, P 〈 0. 05 ） ;IM, AH group, GC group Hp posi- tive patients with gastric mucosa cell c-myc mRNA expression were higher than Hp negative patients （P 〈 0. 05 or 0. 01 ）. Conclusion In the process of the gastric mucosal canceration, Hp infection can increase gastric mucosa cell c-myc mRNA expression, and inhibition of special-shaped programmed cell death （ ap- optosis） and play a role of cancer.

关 键 词：C-MYC MRNA 抑癌基因 程序性死亡 

分 类 号：R735.2[医药卫生—肿瘤]