温度和碳源对隐甲藻生长及DHA积累的影响  

Effects of temperature and carbon source on growth and docosahexaenoic acid accumulation of Crypthecodinium cohnii

作　　者：郭小婧[1] 龚阳敏[1] 梁焯[1] 万霞[1] 胡传炯[1,2] 刘姣[1] 江木兰[1,2] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2013年第5期597-603,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31270346);国家863计划(2011AA100904)

摘　　要：研究了隐甲藻(Crypthecodinium cohnii ATCC 30772)以摇瓶发酵培养方式获得最大生物产量和最高二十二碳六烯酸(docosahexaenoic acid,DHA)产量的培养条件。结果表明,有利于隐甲藻生长和DHA积累的最佳培养条件为:培养温度为22℃,先以12g/L葡萄糖为碳源培养4d,后再以3g/L醋酸钠为碳源培养4d。由此获得的生物产量为234.85mg/(L·d),DHA产量为17.45mg/(L·d),DHA含量为74.36mg/g菌体。Culture conditions were adapted to obtain larger biomass and higher docosahexaenoic acid （DHA） yield of Crypthecodinium cohnii under flask fermentation level.Results showed that the optimal culture should be at 22℃,in carbon-source culture with 12g/L glucose for 4d and then shifted to the same media but with 3g/L sodium acetate instead for 4d.Under this optimal condition,the C.cohnii biomass yield,DHA yield and DHA content reached 234.85mg/（L · d）,17.45mg/（L · d）,74.36mg/g dry weight respectively.

关 键 词：隐甲藻 培养条件 生物量 DHA 

分 类 号：Q93[生物学—微生物学]