蛋白激酶B抑制剂哌立福新对食管癌细胞侵袭影响  

Effects of Akt Inhibitor Perifosine on Migration of Esophageal Carcinoma Cells

作　　者：金灵莉[1] 杜勇[1] 杨艳果[1] 周春芳[1] 吕飞[1] 卢光新[1] 

机构地区：[1]湖北医药学院附属人民医院消化内科,湖北十堰442000

出　　处：《医学新知》2013年第5期336-338,共3页New Medicine

摘　　要：目的探讨蛋白激酶B（Akt）抑制剂哌立福新对食管癌细胞侵袭的作用机制。方法应用哌立福新作用于人食管癌Ecal09细胞株，通过WesternBlotting检测Akt信号通路中相关蛋白Akt、基质金属蛋白-2（MMP-2）基质金属蛋白-9（MMP-2）的表达情况。通过Transwell实验评价食管癌Ecal09细胞侵袭变化情况。结果WesternBlotting结果示：与空白对照组、DMSO组相比，哌立福新组能有效抑制食管癌Ecal09细胞中Akt、MMP-2、MMP-9蛋白的表达。Transwell侵袭实验表明：DMSO组、空白对照组、哌立福新组穿过上室的平均细胞数分别为（81．3±1．52）、（81．0±2．65）、（44．6±2．52），三组的差异具有统计学意义（F=255．1，P〈0．05）。结论哌立福新能有效抑制食管癌Eeal09细胞Akt信号通路中相关蛋白的表达，在体外显著抑制Ecal09细胞的侵袭。Objective To investigate the effects of Akt inhibitor perifosine on migration of esophageal carcinoma cells. Methods Ecal09 cells were treated with perifosine. Expressions of Akt, MMP - 2 and MMP - 9 were detected with western blotting. The effects of perifosine on Ecal09 cell migration were evaluated by Transwell migration experi- ment. Results Perifosine had obvious inhibitory effects on Akt, MMP - 2 and MMP - 9 compared with blank group and DMSO group. Transwell migration experiment showed that average cell numbers through the house in three groups were （ 81.3 ± 1.52 ）, （ 81.0± 2.65 ） and （44.6 ± 2.52 ）. There were significant differences among three groups （ F = 255.1, P 〈 0.05 ）. Conclusion Perifosine can inhibit the expression of related proteins in Akt signaling pathway in Ecal09 cells, and inhibit the migration of Ecal09 cells in vitro.

关 键 词：哌立福新 AKT信号通路 侵袭 MMP-2 MMP-9 

分 类 号：R735.1[医药卫生—肿瘤]