内皮细胞功能的变化与动脉硬化闭塞症发病的关系  

Relationship Between the Changes of Endothelial Cell Function and Pathogenesis of Arteriosclerosis Obliterans

作　　者：李大勇[1] 马贤德[2] 陈文娜[2] 李鑫[1] 李世征[1] 侯俊杰[1] 李雪晶[1] 

机构地区：[1]辽宁中医药大学附属医院血管外科 [2]辽宁中医药大学教学实验中心,辽宁省沈阳市110032

出　　处：《中国动脉硬化杂志》2013年第10期871-875,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(81173273);辽宁省自然科学基金(201202154);辽宁省高等学校优秀人才支持计划(LJQ2012083);沈阳市科学技术项目(F12-277-1-73)

摘　　要：目的探讨内皮细胞功能的变化与动脉硬化闭塞症发病的关系。方法采用高脂饮食喂养及动脉内膜损伤的方法制作大鼠动脉硬化闭塞症模型,分别于造模后4、8、12周用激光多普勒血流仪观测肢体血流灌注,光镜、透射电镜观察动脉大体形态,Percoll密度梯度离心法测定循环内皮细胞数量,酶联免疫吸附双抗体夹心法检测血清中肿瘤坏死因子α、白细胞介素1、内皮素1和一氧化氮水平。结果成功建立了大鼠动脉硬化闭塞症模型,造模后各组大鼠左后肢血流灌注量明显下降,外周血中循环内皮细胞数量明显增多,血清肿瘤坏死因子α、白细胞介素1、内皮素1水平均较正常对照组明显升高,一氧化氮水平较正常对照组明显降低(P<0.01)。结论内皮细胞的损伤及分泌功能的异常是动脉硬化闭塞症发生的重要病理过程,保护血管内皮细胞,改善血管内皮细胞的分泌功能,应是动脉硬化闭塞症早期重要的治疗原则。Aim To discuss the relationship between the changes of endothelial cell function and pathogenesis of arteriosclerosis obliterans （ASO）. Methods ASO models were constructed with the method of high-fat diet plus intimal injury,at 4,8 and 12 weeks after operation,the limb blood perfusion was observed using laser doppler flowmetre,the arterial morphology was observed using light microscopy and transmission electron microscopy,using percoll density gradient centrifugation method to determine the number of circulating endothelial cells （CECs）,serum tumor necrosis factor-α （TNF-α）,interleukin-1 （IL-1）,endothelin-1 （ET-1） and nitric oxide （NO） levels were detected using enzyme linked immunosorbent assay （ELISA） double antibody sandwich assay. Results Rat models of ASO were established successfully,the left limb blood perfusion was significantly decreased after operation,the serum TNF-α,IL-1,ET-1 levels were significantly higher,and the level of NO was significantly less than normal control after operation,and there was significant difference （P〈0.01 ）. Conclusions The injury of endothelial cells and its secretory function is the important pathological process of ASO,and protecting vascular endothelial cells,improving endothelial cell secretion function,should be important therapeutic principles in early ASO.

关 键 词：循环内皮细胞 动脉硬化闭塞症 血流灌注 白细胞介素1 肿瘤坏死因子Α 

分 类 号：R363[医药卫生—病理学]