转化生长因子β受体Ⅰ及Smad4蛋白在肾癌中的表达及意义  

Expression and clinical significance of transforming growth factor β receptorⅠand Smad4 in renal carcinoma

作　　者：张爱莉[1] 任宗涛[1] 

机构地区：[1]河北医科大学第四医院泌尿外科,石家庄050019

出　　处：《临床泌尿外科杂志》2013年第11期819-821,825,共4页Journal of Clinical Urology

摘　　要：目的:探讨转化生长因子β受体Ⅰ(TGF-βRⅠ)及Smad4在肾透明细胞癌(RCCC)及癌旁组织中的蛋白表达情况及临床意义。方法:采用免疫组织化学P-V法分别检测58例RCCC组织、36例癌旁组织中TGF-βRⅠ和Smad4蛋白表达情况,并分析其表达水平与RCCC临床病理特征的关系。结果:①TGF-βRⅠ蛋白在RCCC、癌旁组织的阳性表达率分别为51.7%、80.6%,差异有统计学意义(P<0.05);Smad4蛋白在RCCC、癌旁组织的阳性表达率分别为56.9%、86.1%,差异有统计学意义(P<0.05)。②TGF-βRⅠ和Smad4蛋白在RCCC组织中的表达均与性别、年龄无关(P>0.05),而与肿瘤病理分级及临床分期相关(P<0.05)。③TGF-βRⅠ和Smad4蛋白表达呈正相关。结论:①TGF-βRⅠ、Smad4在RCCC中的表达降低,提示TGF-βRⅠ、Smad4的表达缺失可能参与了RCCC的发生、发展。②TGF-βRⅠ与Smad4之间存在正相关,说明这两种因子在TGF-β信号通路中相互作用,共同参与了RCCC的发生、发展及转移。Objective： To investigate the expression of transforming growth factor β receptor I （TGF-βRI ） and Smad4 in specimens of renal clear cell carcinoma （RCCC） and peritumoral tissues and to study their clinical significance. Method： Immunohistochemistry was used to investigate the protein expression of TGF-βRI and Smad4 in 58 cases of RCCC and 36 cases of peritumoral tissues and their relationship with the clinical pathologic characteristics of RCCC was analyzed. Result： ①The positive expression rates of TGF-βRI in RCCC and peritu-moral tissues were 51.7%, 80.6% respectively, which showed a statistical difference （P〈0.05）. The positive expression rates of Smad4 in RCCC and peritumoral tissues were 56.9 %, 86.1 % respectively, which was a statis-tical difference （P〈0.05）. ②The expressions of TGF-βRI and Smad4 protein were correlated with neither gen-der nor age （P〉0.05）, whereas they were closely associated with clinical stages and pathologic grades （P〈 0.05）. ③There existed a positive correlation between the expression of TGF-βRI and Smad4. Conclusion： ① Lower expression levels of TGF-βRI and Smad4 may be contributed to formation and progression of RCCC. ② TGF-βRI positively correlates with Smad4. Moreover, their interaction may contribute to formation, invasion and metastasis of RCCC.

关 键 词：肾透明细胞癌 转化生长因子Β受体I SMAD4蛋白 免疫组织化学 

分 类 号：R737.11[医药卫生—肿瘤]