不同动物种源肺炎克雷伯氏菌分离株23S rRNA序列分析  

Sequence analysis of 23S rRNA genes of different animal species Klebsiella pneumoniae

作　　者：钟世勋[1] 王迪[1] 曲亭合[1] 潘德琴[1] 黄璇[1] 李奕芙[1] 邵明旭[1] 朱瑞良[1] 

机构地区：[1]山东农业大学动物科技学院山东省动物生物技术与疫病防治重点实验室,山东泰安271018

出　　处：《中国预防兽医学报》2013年第11期937-939,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(30972183)

摘　　要：为分析动物种源肺炎克雷伯氏菌分离株（K．p妇）的进化特点，本研究通过PCR扩增不同动物种源K．pn的23SrRNA基因片段，克隆测序，进行BLAST比对；构建K-pm菌株的系统进化树。结果显示，本实验室保存的12株K．p力核苷酸序列与GenBank收录的X87284株K-pm核苷酸序列同源性为96．8％～99．0％，而与沙门氏菌和奇异变形杆菌同源性只有90．0％～92．7％；8株毛皮动物K．加核苷酸序列同源性为98．1％～99．9％，与猪源、鸡源K．p力的核苷酸序列同源性分别为95．5％～98．1％、96．7％～97．9％；猪源与鸡源K．pn的核苷酸序列同源性为95．9％～97．4％。结果表明，不同动物种源K-pn23SrRNA基因序列同源性差异明显，具有种属的特异性，23SrRNA基因序列可以作为鉴定K-pm的一种快速、简便的方法。To assess the evolution of Klebsiella pneumoniae （K.pn） by 23S rRNA genes, the fragments of 23S rRNA genes were amplified by PCR from different K.pn strains in animal species and sequenced. The alignment analysis results showed that the homologies between twelve K.pn strains were 96.8%-99.0% with the reference strains available in GenBank, which were 90.8%-91.7% with Salmonella and Proteus mirabilis; Of which the homologies between eight fur animal K.pn strains were 98.1% -99.9%, 95.5%-98.1% with porcine K.pn strains, and 96.7%-97.9% with fowl K.pn strains. The homologies between porcine K.pn and fowl K.pn strains were 95.9%-97.4%. The different animal species K.pn strains had a certain degree of genetic difference. 23S rRNA sequence analyses might be a reliable and rapid way for identification of different animal species K.pn.

关 键 词：肺炎克雷伯氏菌 23S RRNA 同源性 进化 基因分析 

分 类 号：S852.61[农业科学—基础兽医学]