杂交竹梢枯病菌产毒诱导因子  

Inductive Condition of Toxin Produced from Arthrinium phaeospermum

作　　者：李姝江[1] 朱天辉[1] 

机构地区：[1]四川农业大学,雅安625014

出　　处：《东北林业大学学报》2013年第11期104-109,共6页Journal of Northeast Forestry University

基　　金：国家自然科学基金项目(31070578)

摘　　要：以杂交竹梢枯病菌为研究对象,在运用单因素筛选最佳培养基的基础上,采用正交试验法对诱导产毒的条件(温度、时间、pH、光照、瓶装量、接种量)进行优化,得到的最优诱导方案为:温度25℃,pH=7.0,黑暗条件下,4块菌丝块(5 mm)接种于120 mL改良Fries+杂交竹煎汁培养液(乳糖等量代替葡萄糖)振荡培养15 d。在此诱导方案下,杂交竹梢枯病菌产毒能力显著提高(P<0.05)。On the basis of the best culture medium by the univariate analysis, the toxin production conditions of Arthrinium phae- ospermum （temperature, time, pH, light, bottle volume and inoculation amount） were studied by orthogonal test. The op- timum inductive plan are as below： four hyphal mass （5 mm） was inoculated in the culture medium （modified Fries＋juice of hybrid bamboo） of 120 mL （ glucose was replaced with equality lactose） with pH of 7.0 and shakily cuhured for 15 days under dark at 25 ℃. The toxin production ability of A. phaeospermum can enhance significantly（P〈0. 05）.

关 键 词：梢枯病菌 诱导条件 真菌毒素 正交试验 杂交竹 

分 类 号：S763.15[农业科学—森林保护学]