藤茶的HPLC指纹图谱及化学模式识别研究  

HPLC Fingerprint and Chemical Pattern Recognition Method of Ampelopsis grossedentata

作　　者：苏素娇[1] 李娜[1] 陈亮[1] 林培玲[2] 丁春花 陈诗强[1] 梁一池[1] 

机构地区：[1]福建中医药大学药学院,福州350122 [2]漳州卫生职业学院,福建漳州363000 [3]西岸生物科技有限公司,福建宁德355300

出　　处：《中国实验方剂学杂志》2013年第22期144-147,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：福建省科技厅社发处重大项目(2011Y4005)

摘　　要：目的:建立藤茶药材HPLC指纹图谱,并进行主成分聚类分析,为其质量控制提供依据。方法:采用UltimateXBC18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.5%甲酸溶液梯度洗脱,检测波长365 nm;柱温40℃;应用SPSS软件对数据进行统计学分析。结果:建立了藤茶药材HPLC指纹图谱共有模式,确定了11个共有峰,并指认了2个主要共有峰。通过主成分聚类分析,18个藤茶不同无性系种质可分为3类。结论:该方法快速,可较全面的反映藤茶药材中化学成分的信息,为评价藤茶药材提供了科学实验依据。Objective：To establish an HPLC fingerprint of Ampelopsis grossedentata,and to make evaluation by principal component and cluster analysis for its quality control.Method：The HPLC analysis was performed on a UltimateXB-C18 column （4.6 mm × 250 mm,5 μm） with column temperature at 40 ℃.The mobile phase was consisted of water containing 0.5 % formic acid and acetonitrile in gradient mode.The detection wavelength was set at 365 nm.All data were analyzed with the SPSS statistical software.Result：The mutual mode of HPLC fingerprint has been established.There were 11 common peaks,two of which were identified.18 samples from different clonal germplasm were classified into three groups based on principal component and cluster analysis.Conclusion：The method was time-saving and represent the chemical information and provide a scientific basis for quality control of Ampelopsis grossedentata.

关 键 词：藤茶 高效液相色谱指纹图谱 主成分分析 聚类分析 

分 类 号：R284.1[医药卫生—中药学]