荔枝核总黄酮对肝纤维化大鼠肝组织Toll样受体2、4表达的影响  

Effects of total flavone of litchi chinensissonn on the expression of toll- like receptor 2 and 4 in rats with hepatic fibrosis

作　　者：何志国[1] 赵永忠[1] 卢青[1] 周英琼[2] 肖绪华[1] 刘 黄大健[1] 成秋宸[1] 

机构地区：[1]桂林医学院附属医院消化内科,广西桂林541004 [2]桂林医学院附属医院病理科,广西桂林541004

出　　处：《广东医学》2013年第19期2926-2930,共5页Guangdong Medical Journal

基　　金：广西自然科学基金资助项目(编号:2012GXNSFAA053105);广西教育厅科研资助项目(编号:201012MS172)

摘　　要：目的探讨荔枝核总黄酮(TFL)对胆管阻塞型肝纤维化大鼠肝组织Toll样受体(TLR)2、TLR4表达的影响。方法雄性SD大鼠80只,随机分为正常对照组(A组)、模型组(B组)、TFL小剂量组(C组)、TFL大剂量组(D组)。除A组外,其余各组均采用胆总管结扎法制备肝纤维化大鼠模型。在造模后第2天开始,A、B组给予生理盐水灌胃,C、D组分别给予100、200 mg/(kg·d)的TFL灌胃,均1次/d,共4周,第4周末处死全部大鼠,留取肝脏组织。HE、Masson染色观察大鼠肝组织病理学改变,免疫组化法及免疫印迹法检测TLR2、TLR4在4组大鼠肝组织内的表达。结果与B组比较,D组肝组织肝纤维化程度明显减轻,胶原表达明显减少(P<0.01);D组大鼠肝组织中TLR2和TLR4蛋白明显减少(P<0.01),C组介于B组和D组之间,但与B组比较差异无统计学意义(P>0.05)。结论 TLR2和TLR4蛋白表达增加可能与胆总管结扎大鼠肝纤维化的发生发展有关,200 mg/(kg·d)TFL能抑制肝纤维化大鼠TLR2、TLR4的表达,并改善大鼠肝组织纤维化程度。Objective To investigate the effects of total flavone of litchi chinensissonn（TFL） on the expression of toll - like receptor - 2 （ TLR - 2） and TLR - 4 in rats with hepatic fibrosis. Methods Eighty males SD rats were random- ly divided into four groups ： sham - operated group （ Group A）, bile duct ligation group （ Group B ）, low - dose TFL group （Group C）, and high -dose TFL group （Group D）. The liver fibrosis rat model was induced by common bile duct ligation （CBDL）. TFL lavage was given to subjects in Group C and D, while replacing saline was givento those in Group A and B from the 2nd day of operation for 4 weeks. Histopathological changes in the livers were studied by hematoxylin - eo- sin （HE） staining and massontrichrome （MT） examination. The expression of TLR- 2 and TLR- 4 in liver tissues was assessed. Results The collagen intensity score and area in Group D was significantly lower than those in Group B （ P 〈 0. 01 ） , with significantly lower expressions of TLR - 2 and TLR - 4 （ P 〈 0. 01 ）. Conclusion The up - regulation of TLR -2 and TLR -4 is correlated with the pathogenesis of CBDL - induced liver fibrosisrats, which could alleviated by high dose TFL.

关 键 词：荔枝核总黄酮 肝纤维化 TOLL样受体2 TOLL样受体4 

分 类 号：R575.2[医药卫生—消化系统]