黄连素、大黄素、五味子及黄芩苷对幽门螺杆菌多重耐药株的体外抑菌作用  

In vitro bacteriostatic effect of berberine, emodin,schisandra, and baicalin on multidrug resistant strains of Helicobacter pylori

作　　者：吴明慧[1] 黄衍强[2] 黄赞松[3] 周喜汉[3] 喻文强[3] 苏建伟[3] 

机构地区：[1]广西医科大学研究生学院,广西壮族自治区南宁市530021 [2]右江民族医学院微生物学和免疫学教研室,广西壮族自治区百色市533000 [3]右江民族医学院消化疾病研究所附属医院消化内科,广西壮族自治区百色市533000

出　　处：《世界华人消化杂志》2013年第30期3247-3251,共5页World Chinese Journal of Digestology

基　　金：广西自然科学基金资助项目;No.2012GXNSFAA053172;广西教育厅重点课题基金资助项目;No.201202ZD078;广西卫生厅课题基金资助项目;No.Z2010082~~

摘　　要：目的:研究黄连素、大黄素、五味子、黄芩苷4种中药成分对幽门螺杆菌(Helicobacter pylori,H.pylori)多重耐药(multiple drug resistance,MDR)株的体外抑菌作用,为临床上根除MDR株提供实验和理论依据.方法:临床分离培养46例H.pylori,琼脂二倍稀释法测定阿莫西林、克拉霉素、左氧氟沙星、四环素、甲硝唑5种抗生素对临床分离株的最低抑菌浓度(minimum inhibitory concentration,MIC),筛选出MDR株;试管二倍稀释法检测4种中药成分黄连素、大黄素、五味子、黄芩苷对MDR株的MIC,比较分析不同中药对H.pylori多重耐药性的影响.结果:共筛选出6株MDR株,4种中药成分对不同的菌株的MIC值不完全相同,其范围分别为:黄连素12.5 mg/mL,大黄素MIC50-200 mg/mL,黄芩苷25-200 mg/mL,五味子50-100 mg/mL.结论:4种中药成分黄连素、大黄素、五味子、黄芩苷对体外MDR株均有一定的抑菌作用,其中黄连素效果更显著.AIM： To examine the in vitro bacteriostatic effect of berberine, emodin, schisandra, and baicalin on multiple drug resistant （MDR） strains of Helicobacter pylori （H. pylori） to provide an experimental basis for clinical killing of these MDR strains. METHODS： After culturing and isolating forty-six H. pylori strains, the minimum inhibitory concentrations （MICs） of amoxicillin, clarithromycin, levofloxacin, tetracycline and metronidazole for the isolated strains were determined by agar dilution method to screen MDR strains. The MICs of berberine, emodin, schisandra, and baicalin for MDR strains were then determined. RESULTS： Six MDR strains were identified. The MICs of berberine, emodin, schisandra, and baicalin for different MDR strains were not identical, and their ranges were： berberine 12.5 mg/mL, emodin 50-200 mg/mL, baicalin 25-200 mg/mL, and schisandra 50-100 mg/mL. CONCLUSION： Each of berberine, emodin, schisandra, and baicalin has an appreciated inhibitory effect on MDR strains in vitro, and the effect of berberine is more significant.

关 键 词：幽门螺杆菌 多重耐药 中药 抑菌 

分 类 号：R285.5[医药卫生—中药学]