先天性膈疝大鼠模型中孕晚期miR-33表达水平分析  

vExpression level of miR-33 in the late stages of Nitrofen-induced congenital diaphragmatic hernia rat

作　　者：朱士博[1] 欧志英[1] 何秋明[1] 钟微[1] 余家康[1] 夏慧敏[1] 

机构地区：[1]广州医学院附属广州市妇女儿童医疗中心,510623

出　　处：《中华小儿外科杂志》2013年第11期854-857,共4页Chinese Journal of Pediatric Surgery

摘　　要：目的研究Nitrofen诱导先天性膈疝（CDH）模型与正常大鼠胎肺中微小RNA（miRNA）的差异表达，寻找与大鼠CDH形成相关的miRNAs并用软件预测其靶基因。方法利用miRNA芯片检测Nitrofen诱导CDH大鼠模型及正常对照组大鼠胎肺中miRNAs的表达并对其表达谱进行差异性分析；挑选芯片结果中CDH组显著下调的miR-33并采用qRT-PCR进行验证；运用生物信息学软件预测可能调控的靶基因。结果通过对CDH和正常大鼠胎肺中miRNA表达谱的差异分析，CDH组中8个上调如miR-3588、miR-382＊等，9个下调如miR-33、miR-193等。qRT-PCR检测显示，miR-33在CDH组中约为正常组的0．64倍（P〈0．05），并对其进行靶基因预测，分别筛选出10个靶基因。结论miR-33在CDH组中表达显著下调，可能通过调控其靶基因而参与实验动物CDH味发育不良的发病机制。Objective To study the differences in expression of microRNAs （miRNAs） between nitrofen induced CDH and normal rat, and to predict the abnormal expression of miRNA target genes. Methods miRNAs expression of 4 normal lung tissues, 4 CDH lung tissues were detected with miRNA microarray chips and the expression level of miR-33 was confirmed by real-time PCR. Potential miRNA targets were analyzed by bioinformatics. Results The results showed that 8 miRNAs were significantly up-regulated （such as miR-3588, miR-382 ＊, etc. ）, and 9 miRNAs down-regulated （such as miR-33,miR-193,etc）. The expression of miR-33 was significantly reduced by 36% in CDH rats when compared to normal tissue （P〈0.05）. Ten potential target genes of miR-33 were predicted by informatics analysis. Conclusions miR-33 may be involved in the pathogenesis of pulmonary hypoplasia of CDH rat by regulating its target genes.

关 键 词：微RNAS 先天性膈疝 支气管肺发育不良 

分 类 号：R-332[医药卫生]