异丙酚对急性肝衰竭小鼠肝损伤的影响  

Effect of propofol on liver injury in mice with acute liver failure

作　　者：李小刚[1] 景桂霞[1] 周荣胜[1] 朱宇麟[1] 李锋[1] 

机构地区：[1]710061西安交通大学医学院第一附属医院麻醉科

出　　处：《中华麻醉学杂志》2013年第9期1131-1133,共3页Chinese Journal of Anesthesiology

基　　金：陕西省科学技术研究发展计划项目资助（2012kw-42-02）

摘　　要：目的 评价异丙酚对急性肝衰竭小鼠肝损伤的影响.方法 成年雄性ICR小鼠80只,1月龄,体重20 ～ 25 g,采用随机数字表法分为3组（n=20）：对照组（Ⅰ组）、急性肝衰竭组（Ⅱ组）和异丙酚组（Ⅲ组）.采用腹腔注射D-氨基半乳糖和LPS的方法制备小鼠急性肝衰竭模型,Ⅲ组腹腔注射D-氨基半乳糖和LPS后6h内,每隔1h尾静脉注射异丙酚5 mg/kg,其余组相应时点注射等容量生理盐水.腹腔注射D-氨基半乳糖和LPS后1、3和6 h（T1-3）时采尾静脉血样,测定血清AST和ALT活性,ELISA法测定血清TNF-α、IL-1β和IL-10浓度；腹腔注射D-氨基半乳糖和LPS后12 h观察小鼠生存情况,采用颈椎脱臼法处死存活小鼠,取肝组织观察病理学结果.结果 与Ⅰ组比较,Ⅱ组和Ⅲ组各时点血清AST和ALT活性、Ⅱ组T1,2时血清TNF-α浓度、各时点血清IL-1β和IL-10浓度,Ⅲ组T1时血清TNF-α浓度、T2,3时血清IL-1β和IL-10浓度升高（P＜0.05）；与Ⅱ组比较,Ⅲ组各时点血清AST和ALT活性、T1,2时血清TNF-α浓度、T2,3时血清IL-1β和IL-10浓度降低,腹腔注射后12 h小鼠生存率升高（P＜0.05）.Ⅱ组和Ⅲ组肝组织病理学损伤程度依次减轻.结论 异丙酚可通过抑制炎性反应减轻急性肝衰竭小鼠的肝损伤.Objective To evaluate the effect of propofol on liver injury in mice with acute liver failure （ALF）.Methods Eighty adult male ICR mice,aged 1 months,weighing 20-25 g,were randomly divided into 3 groups （n =20 each） using a random number table：control group （group Ⅰ）,ALF group （group Ⅱ）,and ALF ＋ propofol group （group Ⅲ）.ALF model was established with intra-peritoneal D-galactosamine （D-GaIN） and lipopolysaccharide （LPS）.Propofol 5 mg/kg was injected via the tail vein every 1 h within 6 h after injection of DGaIN and LPS in group Ⅲ,while the equal volume of normal saline was given instead in the other groups.Venous blood samples were taken from the tail vein at 1,3 and 6 h after injection of D-GaIN and LPS （T1-3） to detect the activities of serum aspartate aminotransferase （AST） and alanine aminotransferase （ALT） and serum tumor necrosis factor-alpha （TNF-α）,interleukin-1β （IL-1β） and IL-10 concentrations （by ILISA）.The survival within 12 h after injection of D-GaIN and LPS was observed and the survival rates were calculated.The mice were sacrificed and livers were removed for microscopic examination of pathologic changes.Results Compared with group Ⅰ,the activities of AST and ALT were significantly increased at each time point in Ⅱ and Ⅲ groups and the serum TNF-α concentrations at T1,2 and IL-1β and IL-10 concentrations at each time point were significantly increased in group Ⅱ,and the serum TNF-α concentrations at T1,and IL-1β and IL-10 concentrations at T2,3 were significantly increased in group Ⅲ （P 〈 0.05）.Compared with group Ⅱ,the activities of AST and ALT at each time point,serum TNF-α concentrations at T1,2 and IL-1β and IL-10 concentrations at T2,3 were significantly decreased and the survival rate within 12 h after injection of D-GaIN and LPS was increased in group Ⅲll （P 〈 0.05）.The pathologic changes of liver tissues were gradually attenuated in Ⅱ and Ⅲ groups.Conclusion Propofol can reduce the liver

关 键 词：二异丙酚 蛋白酶抑制剂 肝功能衰竭 急性 

分 类 号：R575.3[医药卫生—消化系统]