Effects of Acetosyringone on Genetic Transformation of Waxy Maize Using Different Receptor Systems  

作　　者：Xinmei GUO Xinming CHE Yuhe PEI Xiyun SONG 

机构地区：[1]College of Agronomy and Plant Protection,Qingdao Agricultural University [2]Shandong Key Laboratory of Maize Breeding and Cultivation Techniques/Shandong Denghai Seeds Co. ,Ltd.

出　　处：《Agricultural Biotechnology》2013年第5期1-3,8,共4页农业生物技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31201218);National Science and Technology Project of "Twelfth Five Year" Plan of China(2011BAD35B01);Taishan Scholars Program of Shandong Province(Crop Cultivation and Breeding);Basic Research Program of Science and Technology Project of Qingdao City[12-1-4-5-(11)-jch];Starting Fund for High-level Personnel of Qingdao Agricultural University(No.630740);Shandong Key Laboratory of Maize Breeding and Cultivation Techniques

摘　　要：Acetosyringone （AS） has great influences on Agrobacterium-mediated genetic transformation. In order to improve the transformation frequency of waxy maize, in this study, shoot tips and immature embryo-derived callus of Lainongnuo 38 were collected as receptor materials, to compare the effects of pH in suspension medium, addition modes and concentrations of acetosyringone on genetic transformation of waxy maize. Results showed that adding 2 μl of 150 μmol/L AS in the wound with suspension medimn at pH 5.2 can significantly improve the transformation frequency of shoot tips of waxy maize; in genetic transformation of maize callus, adding 5 mg/L AS in infection solution can significantly improve the percentage of resistant callus.Acetosyringone （AS） has great influences on Agrobacterium-mediated genetic transformation. In order to improve the transformation frequency of waxy maize, in this study, shoot tips and immature embryo-derived callus of Lainongnuo 38 were collected as receptor materials, to compare the effects of pH in suspension medium, addition modes and concentrations of acetosyringone on genetic transformation of waxy maize. Results showed that adding 2 μl of 150 μmol/L AS in the wound with suspension medimn at pH 5.2 can significantly improve the transformation frequency of shoot tips of waxy maize; in genetic transformation of maize callus, adding 5 mg/L AS in infection solution can significantly improve the percentage of resistant callus.

关 键 词：Receptor system ACETOSYRINGONE Addition mode Transformation frequency 

分 类 号：S513[农业科学—作物学]