Natural Science Foundation of Hebei Province(C2017301071);"Science and Technology Innovation Project"of Hebei Academy of Agriculture and Forestry Sciences(F18C10002).
[Objectives]The effect of acetosyringone seed soaking on the transformation of maize seed buds was analyzed,so as to improve the genetic transformation efficiency of maize and to provide technical support for transgen...
Solanum lycopersicum‘Heinz 1706’is a pioneer model cultivar for tomato research,whose whole genome sequence valuable for genomics studies is available.Nevertheless,a genetic transformation procedure for this cultiva...
Supported by National Natural Science Foundation of China(31201218);National Science and Technology Project of "Twelfth Five Year" Plan of China(2011BAD35B01);Taishan Scholars Program of Shandong Province(Crop Cultivation and Breeding);Basic Research Program of Science and Technology Project of Qingdao City[12-1-4-5-(11)-jch];Starting Fund for High-level Personnel of Qingdao Agricultural University(No.630740);Shandong Key Laboratory of Maize Breeding and Cultivation Techniques
Acetosyringone (AS) has great influences on Agrobacterium-mediated genetic transformation. In order to improve the transformation frequency of waxy maize, in this study, shoot tips and immature embryo-derived callus...
So far there is no suitable vector system for the genetic engineering of monocotyledonous plants, especially cereal crops. It is first reported in this paper that Agrobacterium tumefaciens strains T37, A208 and B6 can...
Agrobacterium tumefaciens can infect wounded plant cells, transfer a part of its Ti plasmid (T-DNA) into plant genomes and cause crown gall tumors. At present the Ti plasmid of A. tumefaciens is the best vector availa...