云南高校图书馆联盟文献共享服务平台- TUMEFACIENS

TUMEFACIENS: 作品数：33被引量：48H指数：4; 导出分析报告; 相关领域：农业科学生物学更多>>; 相关作者：李敏慧姜子德曾大兴康霞徐刚更多>>; 相关机构：华南农业大学深圳职业技术学院甘肃农业大学西北农林科技大学更多>>; 相关期刊：《Oil Crop Science》《Science China(Life Sciences)》《Science China Chemistry》《Advances in Microbiology》更多>>; 相关基金：国家自然科学基金甘肃省自然科学基金中央级公益性科研院所基本科研业务费专项国家高技术研究发展计划更多>>

Development of an Agrobacterium tumefaciens-mediated transformation system for somatic embryos and transcriptome analysis of LcMYB1's inhibitory effect on somatic embryogenesis in Litchi chinensis: 《Journal of Integrative Agriculture》2025年第2期594-609,共16页Yaqi Qin Bo Zhang Xueliang Luo Shiqian Wang Jiaxin Fu Zhike Zhang Yonghua Qin Jietang Zhao Guibing Hu; supported by the National Natural Science Foundation of China(31872066 and 32272663);the Science and Technology Planning Project of Guangzhou,China(2023B01J2002);the Key Research and Development Program of Hainan,China(ZDYF2023XDNY052);the Seed Industry Engineering Project of Department of Agriculture and Rural Affairs of Guangdong,China(2022-NPY-00-004 and 2022-NBH00-001);the Litchi Industry Science and Technology Special Mission of Yunnan,China(202204BI090021)。; Litchi has great economic significance as a global fruit crop.However,the advancement of litchi functional genomics has encountered substantial obstacles due to its recalcitrance to stable transformation.Here,we prese...; 关键词：Litchi chinensis A.tumefaciens TRANSFORMATION LcMYB1 somatic embryo

Establishing VIGS and CRISPR/Cas9 techniques to verify RsPDS function in radish被引量：1: 《Journal of Integrative Agriculture》2024年第5期1557-1567,共11页Jiali Ying Yan Wang Liang Xu Tiaojiao Qin Kai Xia Peng Zhang Yinbo Ma Keyun Zhang Lun Wang Junhui Dong Lianxue Fan Yuelin Zhu Liwang Liu; This work was supported by Jiangsu Seed Industry Revitalization Project,China[JBGS(2021)071];Fundamental Research Funds for the Central Universities,China(YDZX2023019);the National Natural Science Foundation of China(32172579);the earmarked fund for Jiangsu Agricultural Industry Technology System,China[JATS(2023)421];the Jiangsu Postgraduate Scientific Research Innovation Plan,China(KYCX21_0610-2021);the Project Founded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD).; Virus-induced gene silencing(VIGS)and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)systems are effective technologies for rapid and accurate gene function verification...; 关键词：Raphanus sativus L. VIGS CRISPR/Cas9 Agrobacterium rhizogenes A.tumefaciens RsPDS

Expression of a Rice Chitinase Gene Enhances Antifungal Response in Transgenic Litchi (cv. Bedana): 《American Journal of Plant Sciences》2018年第11期2256-2275,共20页Dilip Kumar Das Amir Rahman; To enhance the antifungal response of litchi, transferring rice chitinase gene under a maize-ubiquitin promoter along with its first intron into the zygotic embryos via Agrobacterium tumefaciens-mediated transformatio...; 关键词：TRANSGENIC LITCHI AGROBACTERIUM TUMEFACIENS Blight Die Back and Leaf Spots PHOMOPSIS sp. Somatic EMBRYOGENESIS Transformation

Cotyledons:a useful biological material for transient gene expression analysis in rapeseed(Brassica napus L.): 《Oil Crop Science》2018年第1期12-20,共9页Ming Zheng Hongli Yang Min Tang Jinglin Liu Xiaokang Li Liang Zhang Zhiyong Hu Wei Hua; supported by the National KeyBasic Research Program of China (2015CB150200);the Natural Science Foundation of Hubei Province (2016CFB286); Polyethylene glycol (PEG)-mediated DNA transformation for transient gene expression in protoplasts and Agrobacterium tumefaciens-mediated transformation in lower epidermis of leaves are readily available in several pl...; 关键词：RAPESEED COTYLEDON protoplast PEG Agrobacterium TUMEFACIENS YFP GUS TRANSIENT expression

Transformation of Arabidopsis thaliana ESB1 Gene into Agrobacterium tumefaciens and Its Identification: 《Trends in Genetics and Evolution》2018年第1期20-25,共6页Zhongling Gong Wenjie Xu; Botrytis cinerea is a major disease in crop production. Chrysanthemum gray mold is easy to occur during chrysanthemum growing season. It can cause a lot of defoliation when serious, affecting plant flowering and reduc...; 关键词：ESB1 gene AGROBACTERIUM TUMEFACIENS Genetic transformation Cut FLOWER CHRYSANTHEMUM

Arabidopsis NahG Plants as a Suitable and Efficient System for Transient Expression using Agrobacte#um tumefaciens被引量：2: 《Molecular Plant》2017年第2期353-356,共4页; Dear Editor Research in molecular plant biology has largely benefitted from technologies allowing exogenous gene expression. While the generation of stable transgenic lines is required in some cases, transient transfo...

根癌农杆菌介导水稻成熟胚及抗褐飞虱基因植株的获得: 《湖北农业科学》2016年第9期2392-2395,共4页李三和韩光明闸雯俊刘凯胡刚游艾青; 国家转基因重大专项(2014ZX0800101B);"973"计划项目(2013CBA01405); 以水稻(Oryza sativa L.)籼型两系恢复系M5274和晚粳稻不育系N55S成熟胚为材料,以携带有双元载体的根癌农杆菌(Agrobacterium tumefaciens)EHA105为载体进行抗飞虱基因Bph14、Bphi008A、Osg1的遗传转化,共获得515棵再生植株,包括198株Bp...; 关键词：水稻(Oryza sativa L.) 根癌农杆菌(Agrobacterium tumefaciens)介导转化抗褐飞虱基因

根癌农杆菌(Agrobacterium tumefaciens)介导的马铃薯高效遗传转化体系筛选及优化被引量：5: 《中国沙漠》2016年第1期225-231,共7页康霞徐刚王玉萍; 甘肃省高等学校科研项目(1002-07);甘肃省自然科学基金项目(1506RJZA013);甘肃省高校基本科研业务费项目;甘肃省农业厅生物技术专项(GNSW-2008-07); 马铃薯是重要的块茎类作物,根癌农杆菌(Agrobacterium tumefaciens)介导的遗传转化方法是马铃薯基因工程育种的重要技术,研究其遗传转化体系和转化效率有助于提高分子育种效率。针对利用根癌农杆菌介导的2种外植体遗传转化马铃薯普通栽...; 关键词：根癌农杆菌(Agrobacterium tumefaciens) 遗传转化愈伤诱导分化转化率

<i>Agrobacterium</i>-Mediated Transformation of Mexican Lime (<i>Citrus aurantifolia</i>Swingle) Using Optimized Systems for Epicotyls and Cotyledons: 《Advances in Bioscience and Biotechnology》2015年第11期657-668,共12页Maria Luiza P. de Oliveira Gloria Moore James G. Thomson Ed Stover; Transgenic Mexican lime (Citrus aurantifolia Swingle) was produced through two explant sources, each using systems previously optimized for each source. One used epicotyls segments, which was the predominant explant f...; 关键词：AGROBACTERIUM TUMEFACIENS Embryo COTYLEDONS Epicotyls Segments RECOMBINASE Mediated Exchange Cassette Tissue Culture

异养硝化好氧反硝化菌株Agrobacterium tumefaciens LAD9羟胺氧化酶的分离纯化被引量：2: 《生物技术通报》2014年第7期69-73,共5页陈倩马涛王婷; 国家自然科学基金项目(51208007);高等学校博士点新教师类基金项目(20120001120101); 羟氨氧化酶(Hydroxylamine oxidase,HAO)的作用方式直接决定了异养硝化好氧反硝化细菌的代谢途径,分离得到纯度较高的HAO也就成为研究这类细菌脱氮机制的重要环节。以异养硝化好氧反硝化细菌Agrobacterium tumefaciens LAD9为代表,建立...; 关键词：脱氮羟胺氧化酶分离纯化 AGROBACTERIUM TUMEFACIENS LAD9 异养硝化好氧反硝化

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