根癌农杆菌介导水稻成熟胚及抗褐飞虱基因植株的获得  

Agrobacterium-mediated Transformation of Rice Mature Embryos and Regeneration of Transgenic Plants with Brown Planthopper(Nilaparvata lugens St覽l) Resistance Genes

作　　者：李三和[1] 韩光明[1] 闸雯俊[1] 刘凯[1] 胡刚[1] 游艾青[1] 

机构地区：[1]湖北省农业科学院/主要粮食作物产业化湖北省协同创新中心/湖北省作物种质与遗传改良重点实验室,武汉430064

出　　处：《湖北农业科学》2016年第9期2392-2395,共4页Hubei Agricultural Sciences

基　　金：国家转基因重大专项(2014ZX0800101B);"973"计划项目(2013CBA01405)

摘　　要：以水稻(Oryza sativa L.)籼型两系恢复系M5274和晚粳稻不育系N55S成熟胚为材料,以携带有双元载体的根癌农杆菌(Agrobacterium tumefaciens)EHA105为载体进行抗飞虱基因Bph14、Bphi008A、Osg1的遗传转化,共获得515棵再生植株,包括198株Bph14转基因植株、72株Bphi008A转基因植株和245株Osg1转基因植株。PCR检测结果表明,获得的515株再生植株中,有244株阳性转基因植株,3个不同抗褐飞虱基因中,转Bph14基因的再生苗阳性率最高,增加筛选次数能有效减少转化所得的假阳性植株。The mature embryos of an indica restorer line M5274 and late japonica CMS line N55 S of rice（Oryza sativa L.）were used to study on Agrobacterium-mediated transformation for brown planthopper resistance genes, Bph14、Bphi008A and Osg1. 515 regenerated plants were obtained, including 198 Bph14 transgenic plants, 72 Bphi008 A transgenic plants and 245Osg1 transgenic plants. Through PCR analysis on 515 transgenic plants, 244 of them were found to be positive. Transgenic plants with Bph14 gene had a much higher positive ratio than those plants with Bphi008 A or Osg1 gene. This study also indicated that increasing screen time can availably decrease the negative plants in transformation.

关 键 词：水稻(Oryza sativa L.) 根癌农杆菌(Agrobacterium tumefaciens)介导转化 抗褐飞虱基因 

分 类 号：S511[农业科学—作物学]