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作品数：48被引量：105H指数：6
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相关期刊：《Journal of Integrative Agriculture》《Rice science》《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》《Life Research》更多>>
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On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay
《Journal of Integrative Agriculture》2025年第2期790-794,共5页Kaikai Jin Junjie Zhao Huanxin Chen Zimo Zhang Zengguo Cao Zanheng Huang Hao Li Yongsai Liu Lisi Ai Yufei Liu Changqi Fan Yuanyuan Li Pei Huang Hualei Wang Haili Zhang 
supported by the National Key Research and Development Program of China(2021YFF0703600)。
Highlights The RT-RAA-VF assay developed for the NiV P gene can perform rapid detection of NiV within 20 min at 42℃ with high specificity.This assay is capable of attaining sensitivity to a single copy of NiV RNA tra...
关键词：Singh destroyed visual 
Dye-based recombinase-aided amplification assay with enhanced sensitivity and specificity
《iLABMED》2024年第4期294-306,共13页Zijin Zhao Yanbo You Shaowei Hua Xinxin Shen Lingjun Li Xuejun Ma 
National Key R&D Program of China,Grant/Award Number:2021YFC2301102;National Natural Science Foundation of China,Grant/Award Numbers:82202593,U23A20106。
Background:Fluorescent recombinase-aided amplification(RAA)assays are increasingly being used in the detection of a variety of pathogens and have the advantages of rapidity and simplicity and similar sensitivity and s...
关键词：EvaGreen dye Listeria monocytogenes melt curve nucleotide analogs raw milk recombinase aided amplification SAMRS Staphylococcus aureus 
Rapid detection of the rice false smut fungus Ustilaginoidea virens by lateral flow strip-based recombinase polymerase amplification assay
《Journal of Integrative Agriculture》2024年第11期3763-3773,共11页Jiacheng Xi Sanlian Wan Yue Li Yuandi Xu Jing Yang Ting Zhang Jiajia Chen Zhengguang Zhang Danyu Shen Haifeng Zhang 
supported by grants from the Jiangsu Agricultural Science and Technology Innovation Fund,China(JASTIF)(CX(21)3012)to Haifeng Zhang。
Rice false smut,caused by Ustilaginoidea virens,is a devastating disease that greatly reduces rice yield and quality.However,controlling rice false smut disease is challenging due to the unique infection mode of U.vir...
关键词：rice false smut Ustilaginoidea virens isothermal amplification disease monitoring field diagnosis 
A recombinase polymerase amplification with lateral flow assay for rapid on-the-spot detection of Aeromonas salmonicida
《Water Biology and Security》2024年第3期58-64,共7页Sijie Zhang Yuanxing Zhang Qin Liu Qiyao Wang Yibei Zhang 
financially supported by National Key Research and Development Program of China(2022YFE0101200);National Natural Science Foundation of China(32102850);Shanghai Agricultural Science and Technology Innovation Project(T2023328).
Aeromonas salmonicida is a common pathogen of salmonid fishes that poses a significant threat to the fresh water and marine culture industry,potentially resulting in huge economic losses.To prevent and control fish di...
关键词：Aeromonas salmonicida Recombinase Polymerase Amplification(RPA) Lateral flow On-the-spot detection 
Development of a multiplex recombinase polymerase amplification coupled with lateral flow dipsticks for the simultaneous rapid detection of Salmonella spp.,Salmonella Typhimurium and Salmonella Enteritidis
《Food Quality and Safety》2024年第2期302-311,共10页Zeqiang Zhan Shoukui He Yan Cui Jinzeng Yang Xianming Shi 
supported by the National Key R&D Program of China(No.2019YFE0119700);the National Natural Science Foundation of China(No.32172316).
Objectives:Salmonella spp.is a world-leading foodborne pathogen and its rapid detection is essential for ensuring food safety.Conventional methods require expensive instruments,considerable operational skills and cann...
关键词：SALMONELLA multiplex detection lateral flow dipstick recombinase polymerase amplification meat product 
A LFD-RAA-Based Rapid Detection Method for Pseudomonas syringae pv. tabaci
《Agricultural Science & Technology》2024年第2期38-43,共6页XIAO Yan-song LI Hong-guang LI Si-jun WU Wen-xin ZHOU Lu-su ZHONG Jie SU Jia-en YANG Zhi-juan 
湖南省郴州市烟草公司烟草农业科技创新项目(CZYC2022JS03,CZYC2023JS05);云南省烟草公司大理州公司科技计划一般项目(DLYC2022003)。
This study aimed to achieve rapid detection of Pseudomonas syringae pv.tabaci,the pathogen of tobacco wildfire disease.The specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as...
关键词：Tobacco wildfire disease Recombinase-aided amplification DETECTION 
Development of RPA-Cas12a-fluorescence assay for rapid and reliable detection of human bocavirus 1被引量：1
《Animal Models and Experimental Medicine》2024年第2期179-188,共10页Weidong Qian Xuefei Wang Ting Wang Jie Huang Qian Zhang Yongdong Li Si Chen 
Natural Science Foundation of China;Grant/Award Number:81973531;Science and Technology Plan Project of Xi’an;Grant/Award Number:22GXFW0007;Shenzhen Science and Technology Innovation Commission;Grant/Award Number:20200812211704001;Medical Scientific Research Foundation of Guangdong Province;Grant/Award Number:A2019502;Nanshan District Science and Technology Plan Project;Grant/Award Number:NS2022022;Scientific Research Program Funded by Shaanxi Provincial Education Department;Grant/Award Number:22JC010
Human bocavirus(HBoV)1 is considered an important pathogen that mainly affects infants aged 6–24 months,but preventing viral transmission in resource-limited regions through rapid and affordable on-site diagnosis of ...
关键词：CRISPR-Cas12a DETECTION human bocavirus 1 on-site diagnosis recombinase polymerase amplification 
Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology被引量：1
《Biomedical and Environmental Sciences》2024年第4期387-398,共12页ZHAO Zi Jin CHEN Xiao Ping HUA Shao Wei LI Feng Yu ZHAO Meng XING Chen Hao WANG Jie TIAN Feng Yu ZHANG Rui Qing LYU Xiao Na HAN Zhi Qiang WANG Yu Xin LI Hong Yi SHEN Xin Xin MA Xue Jun TIE Yan Qing 
funded by the National Key R&D Program of China[2021YFC2301102];National Natural Science Foundation of China[82202593];Key R&D Program of Hebei Province[223777100D].
Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification method.This study aimed to develop an assay that can be used for the early diagnosis of three t...
关键词：Staphylococcus aureus Pseudomonas aeruginosa Acinetobacter baumannii Human Mannan-binding lectin protein Bloodstream infection Recombinase-aided PCR assay Multiple detection 
An exo Probe-based Reverse Transcription and Recombinase Polymerase Amplification for Rapid Detection of Feline Calicivirus in Cats
《Biomedical and Environmental Sciences》2024年第3期325-330,共6页MU Hong Yun ZHOU Hong Lei JING Zheng LU Wei ZHANG Jian Wei 
supported by the natural science foundation reserve project of Jiangsu Agri-animal Husbandry Vocational College(No.NSF2023CB14).
Feline calicivirus(FCV)was a highly prevalent RNA virus causing upper respiratory tract infections in cats through the mouth and nose around the world,result in the death of cats especially under 1 year old with upper...
关键词：prevention RESPIRATORY PROBE 
A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops被引量：1
《Food Science and Human Wellness》2024年第1期183-190,共8页Jinbin Wang Yu Wang Xiuwen Hu Yifan Chen Wei Jiang Xiaofeng Liu Juan Liu Lemei Zhu Haijuan Zeng Hua Liu 
supported by the Scientific and Innovative Action Plan of Shanghai(21N31900800);Shanghai Rising-Star Program(23QB1403500);the Shanghai Sailing Program(20YF1443000);Shanghai Science and Technology Commission,the Belt and Road Project(20310750500);Talent Project of SAAS(2023-2025);Runup Plan of SAAS(ZP22211);the SAAS Program for Excellent Research Team(2022(B-16))。
Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSP...
关键词：Genetically modifi ed crops On-site detection Lateral fl ow test strips Dual recombinase polymerase amplification (RPA) 
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