Effects of Acetylacetone Solution Soaking on Agrobacterium - transformed Maize Seed Buds  

作　　者：Mengyu LYU Fushuang DONG Shuo ZHOU Jianfang CHAI He ZHAO Yongwei LIU Fan YANG Bo JIAO Xiaoyu LI Junmin ZHANG Haibo WANG 

机构地区：[1]Plant Genetic Engineering Center of Hebei Province,Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Sciences(Agricultural Product Quality and Safety Research Center of Hebei Academy of Agricultural and Forestry Sciences),Shijiazhuang 050051,China

出　　处：《Agricultural Biotechnology》2021年第3期11-14,共4页农业生物技术（英文版）

基　　金：Natural Science Foundation of Hebei Province(C2017301071);"Science and Technology Innovation Project"of Hebei Academy of Agriculture and Forestry Sciences(F18C10002).

摘　　要：[Objectives]The effect of acetosyringone seed soaking on the transformation of maize seed buds was analyzed,so as to improve the genetic transformation efficiency of maize and to provide technical support for transgenic breeding of maize.[Methods]The seeds of the"Zheng 58"maize inbred line were used as experimental materials.When the seeds were germinated,AS was added to the water at concentrations of 70,140,210,and 280μmol/L,respectively,and the seeds germinated without the addition of AS served as the CK.The Agrobacterium-mediated method was used to transform bud growth points of maize seeds,and green fluorescent protein detection was performed on the young shoots transformed with EGFP(enhanced green fluorescent protein)gene.The effect of soaking seeds with acetosyringone solution on the transformation of maize bud growth points by Agrobacterium was studied according to the detection results.[Results]Soaking seeds in acetosyringone solutions for germination had the effect of inhibiting the germination of maize seeds and inhibiting sprout elongation,and the higher the concentration of acetosyringone,the stronger the inhibition.When the concentration of acetosyringone solution was 280μmol/L,the germination rate of seeds was only 36.2%of the CK,while soaking seeds with 70-140μmol/L acetosyringone solution for germination could not only ensure a higher germination rate of maize seeds,but also significantly increased the transformation efficiency of maize bud growth points.When the seeds were soaked with 70μmol/L acetosyringone solution for germination,the positive rate of transformed maize buds was the highest,reaching 32.1%.[Conclusions]When maize bud growth points were used as the receptor of Agrobacterium transformation,soaking seeds with 70-140μmol/L acetosyringone for germination basically did not affect the germination of seeds,and was beneficial to the activation of Agrobacterium,thereby promoting the transformation.

关 键 词：Maize ACETOSYRINGONE Seed soaking Transgenic efficiency 

分 类 号：S513[农业科学—作物学]