陕西小麦黄色素含量基因TaZds-A1和TaZds-D1的组成与分布  

Detection of Genes TaZds-A1 and TaZds-D1 for Yellow Pigment Content and Their Distribution in Shaanxi Wheat Cultivars and Lines

作　　者：张钰玉[1] 张晓科[1] 蒋雷[1] 刘芳军[1] 王宪国[1] 白升升[1] 王晓龙[1] 

机构地区：[1]西北农林科技大学农学院/国家小麦改良中心杨凌分中心,陕西杨凌712100

出　　处：《麦类作物学报》2013年第6期1144-1148,共5页Journal of Triticeae Crops

基　　金：小麦产业技术体系建设专项(nycytx-03);948项目(2011-G3);陕西省教育厅项目(08JK212);西北农林科技大学唐仲英育种基金项目

摘　　要：面粉或面制品色泽是小麦重要的品质评价指标,ζ-胡萝卜素脱氢酶(ζ-carotene desaturase,ZDS)活性基因是控制小麦籽粒或面粉中黄色素(Yellow pigment,YP)含量的重要基因。为给陕西小麦品质改良提供参考依据,利用2A和2D染色体上的YP2A1-和YP2D1-标记,对陕西194份小麦品种(系)分别进行检测,分析TaZds-A1和TaZds-D1两位点等位变异的组成和分布特点。结果表明,在陕西小麦中,TaZds-A1位点存在TaZds-A1a和TaZds-A1b两种等位变异类型,分别占43.3%和56.7%;而在TaZds-D1位点,全为TaZds-D1a等位变异类型。陕西小麦两位点存在TaZds-A1a/TaZds-D1a和TaZds-A1b/TaZds-D1a两种等位变异的组合类型,在不同地区小麦中的分布不同:在陕西渭北旱塬和关中地区小麦中,两种组合类型的频率相当,约各占一半;在陕南地区小麦中,高YP含量的TaZds-A1b/TaZds-D1a组合类型占三分之二。ZDS活性基因的选择将成为以后陕西小麦色泽品质遗传改良研究中的一个重要部分。The color of flour or end-use products of wheat is an important quality index for wheat. 4- carotene desaturase （ZDS） genes were demonstrated as the yellow pigment （YP） content in wheat grain to influence the color of wheat flour. In this study, molecular markers YP2A-1 located on chro- mosome 2A and YP2D-1 located on chromosome 2D were used to detect and analyze their distribution characteristics in 194 wheat cultivars and advanced lines in Shaanxi province. At TaZds-A1 locus, two allelic variations TaZds-Ala and TaZds-Alb were distinguished by the codominant marker YP2A-1 , which took up a proportion of 43.3% and 56.7%, respectively. While at TaZds-D1 locus, only one allele TaZds-Dla was found by the molecular marker YP2D-1 . There were two allelic combinations at the two loci in the Shaanxi wheat, and their frequencies were distinct in different wheat regions of Shaanxi. In Weibei plateau and Central Shaanxi plain, the frequencies of two allelic combinations were close, taking up about 50 percent of each. In Southern Shaanxi region, the TaZds-Alb /TaZds-Dla related to high YP content accounted for two thirds. The results indicated that ZDS genes were ig nored in recent wheat quality breeding in Shaanxi province. Selecting the ZDS genes will be an impor tant part of the study on wheat quality breeding.

关 键 词：小麦 面粉色泽 黄色素含量 分布频率 

分 类 号：S512.1[农业科学—作物学] S331